Frozen bacteria were cultured onto Bordet-Gengou Agar
(BGA) (Becton Dickinson, USA) supplemented with 1% glycerol, peptone, and 20% defibrinated sheep blood and were incubated at 35[degrees]C for 72 hr.
([dagger]) MICs were determined by E-test on Bordet-Gengou agar
. ([double dagger]) Site and day (d) of collection of isolate.
A special culture medium that contains activated charcoal (e.g., Regan-Lowe agar) or potato-derived starch (i.e., Bordet-Gengou agar
) and defibrinated horse of sheep blood to neutralize the inhibitory substances must be used to isolate B.
pertussis strains were grown on Bordet-Gengou agar
plates supplemented with 15% sheep blood and incubated for 3 days at 35[degrees]C.