Western blot
(redirected from Blotting, western)Also found in: Dictionary.
Western blot
Immunoblot Molecular diagnostics An assay that IDs antibodies to proteins of specific molecular weights, used to confirm HIV infection–after a positive ELISA screening assay, Lyme disease. See Blot, Electrophoresis, Protein. Cf Northern blot, Southern blot.West·ern blot a·nal·y·sis
(wes'tĕrn blot ă-nal'i-sis)A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]
Western blot
A technique developed in 1979 that is used to confirm ELISA results. HIV antigen is purified by electrophoresis and attached by blotting to a nylon or nitrocellulose filter. The patient's serum is reacted against the filter, followed by treatment with developing chemicals that allow HIV antibody to show up as a colored patch or blot. If the patient is HIV-positive, there will be stripes at specific locations for two or more viral proteins. A negative result is blank.
Mentioned in: AIDS Tests
Western blot,
n.pr a confirmatory test for HIV exposure that identifies antibodies to HIV proteins and glycoproteins.
Western blot
an immunologic technique for the detection of proteins using a radioactive probe. Called also Western transfer.