Confirmation of diagnosis was made by demonstration of plasmodium in the peripheral blood by thin and thick blood film examination by immunochromatographic test, Quantitative Buffy Coat analysis for parasite, complete blood profile including platelet count.
However, before discharge on day 6, thick blood film examination was still positive (192/[micro]L), but by day 11, his repeat blood film examination and his aldolase rapid diagnostic test results were negative.
This was followed by investigations such as complete blood count (CBC) with red cell indices, peripheral blood film (PBF) smear, erythrocyte sedimentation rate, serum iron, total iron binding capacity (TIBC), and serum ferritin.
Interestingly, automated blood film review is now possible using advanced microscopy such as the advanced RBC application of the Cellavision automated microscope which is able to pre-classify and pre-characterise almost all morphologic alterations in red cell morphology before validation .
Beside manual estimation of platelet count, blood film evaluation provides additional assessment of platelet size, shape, granulation, and analysis of phenomena such as aggregation or platelet satellitism.