Still, no cases with blasts were missed by the optimized criteria, although the blast flag was only detected in 11 of 12 cases.
of basophils, >500 NA /[micro]L Basophil, % NA >4 Reticulocyte Absolute reticulocyte, >0.100 NA x [10.sup.3]/[micro]L Suspect flags Nucleated red Flag Flag blood cell Blast Flag Flag Atypical lymphocyte Flag Flag RBC fragment Flag NA Dimorphic RBC Flag NA Lyse resistant Flag NA Immature granulocyte Flag NA Left shift Flag NA PLT clump Flag NA Platelet (except Flag NA PLT clump) Suspect flag (except Flag NA ImmG/band in adult) Suspect flag (child) Flag NA Abbreviations: CBC, complete blood count; HGB, hemoglobin; ImmG, immature granulocyte; MCV, mean corpuscular volume; NA, not available; PLT, platelet; RDW, red cell distribution width; WBC, white blood cell; RBC, red blood cell.
In samples with atypical lymphocytes, 11 of 12 (91.67%) were triggered by PLT counts less than 100 x [10.sup.3][micro]L, 10 of 12 (83.33%) were triggered by atypical lymphocyte flags, and 9 of 12 (75%) were triggered by blast flags (abnormal lymphocytes/ lymphoblast flags).
For example, instruments that use light scatter and fluorescence will flag samples that contain cell populations in certain areas of their scatter-grams with a blast flag. The presence of one or more flags does not indicate that a specific abnormality or any abnormality has to be present; it only indicates that there is an increased probability of an abnormality that can only be excluded or proven by slide review.
Optimization began by raising the cutoff of each flag from the factory default setting in increments of 10 units and calculating the YI of each level for the identification of the specific abnormality denoted by the flag (eg, the blast flag for blasts).
The abnormality-specific PPV of each of the 5 flags was between 5.4% (PPV of the blast flag for the presence of blasts) and 33% (PPV of the immature granulocyte flag for the presence of myelocytes and/or metamyelocytes) (Table 2).
All 14 cases were detected by our optimized criteria; 12 (86%) by the blast flag and 2 (14%) by a combination of the other 4 user-adjustable flags.
No cases of blasts would have been missed using optimized criteria, although the optimized blast flag only detected 12 of the 14 cases.
The last missed case would only have been detected by decreasing the blast flag to 110.
Number of False-Positive Samples and the Positive Predictive Values (PPVs) of Each Flag for Its Specific Abnormal Finding (Optimization Set, n = 502) Immature Category Blast Flag Granulocyte Flag Factory-set thresholds False-positives, No.
Combined evaluation of the Blast flag
with the Abn Lympho/L_Blasts flag on the XE-2100 did not increase the sensitivity of blast cell flagging but resulted in an increase of FP results.