biotinylation

(redirected from Biotin labeling)

biotinylation

The covalent linking of biotin to a protein, peptide, nucleic acid (DNA, RNA) or other biomolecule of interest, which in turn binds with high affinity to avidin or streptavidin linked to an enzymatic moiety or fluorescent molecule. Biotinylation is used in detector systems and to isolate biotinylated biomolecules of interest.
References in periodicals archive ?
The sizes of amplification products and of fragmented and biotin-labeled products prepared for array analysis, after fragmentation and biotin labeling, were determined by Lab-on-a-Chip analysis in a Bioanalyzer (Agilent).
The Ovation Biotin System provides reagents and protocols for fragmentation and biotin labeling of the amplification products.
Enzo's BioArray(TM) RNA Amplification and Labeling System supplies all reagents for the amplification and biotin labeling of antisense RNA for use with eukaryotic gene expression microarrays.
One mg of antibody in PBS was reacted with biotin-7-NHS (D-biotinoyl-[member of]-aminocaproic acid-N-hydroxysuccinimide ester) for 2 h at room temperature by using a biotin labeling kit (Boehringer Mannheim Biochemica, Mannheim, Germany).
PDBA-modified RNA can be used with the full range of Versalinx Tools enabling assays exhibiting high sensitivities and low background and in experiments where endogenous biotin would interfere with detection using biotin labeling (e.