biotinylation

(redirected from Biotin label)

biotinylation

The covalent linking of biotin to a protein, peptide, nucleic acid (DNA, RNA) or other biomolecule of interest, which in turn binds with high affinity to avidin or streptavidin linked to an enzymatic moiety or fluorescent molecule. Biotinylation is used in detector systems and to isolate biotinylated biomolecules of interest.
References in periodicals archive ?
Primer combinations were chosen such that fragments were obtained containing either a single DIG or single biotin label, both labels, or no label at all.
These two labels enable simultaneous binding of PCR products to microparticles coated with anti-digoxigenin antibodies and staining with streptavidin-R-phycoerythrin utilizing the biotin label.
We also demonstrated for the first time that biotin labels attached to diGTP could be site specifically incorporated into extension primers.