Further evaluation of HPA occurring as a secondary consequence of a defect in the metabolism or regeneration of BH4 is done through analysis of urine or blood neopterin, biopterin
and measurement of DHPR activity.
However, inflammation can also oxidate BH4 due to increasing production of ROS, which in turn forms dihydrobiopterin (BH2) and ultimately biopterin
BH4 concentrations, expressed as pmol/mg protein, were calculated by subtracting dihydrobiopterin (BH2) and oxidized biopterin
from the total biopterins
major cocktail vaccine utilized for the inoculation of mice consists of two DNA plasmids, one encoding for the antigen proteins biopterin
transporter (BT) and the intercellular cell adhesion molecules (ICAM), as well as plasmids containing the open reading frame (ORFF), the transmembrane protein Amastin, and PVAX, which is the only plasmid approved by the FDA for human vaccinations due to its unstable properties that allow for its easy removal from the body and minimizes the possibility of chromosomal integration [17-20].
Nixon, "Analysis of reduced forms of biopterin
in biological tissues and fluids," Analytical Biochemistry, vol.
Quantitative regulation of intracellular endothelial nitric-oxide synthase (eNOS) coupling by both tetrahydrobiopterin-eNOS stoichiometry and biopterin
redox status: Insights from cells with tet-regulated GTP cyclohydrolase I expression.
Human monocytes/macrophages lack the enzyme 6-pyruvoyltetra-hydro biopterin
synthase, which converts N[H.sub.2]TP to 6pyruvoyl-tetra-hydro biopterin
Critical role for tetrahydrobiopterin recycling by dihydrofolate reductase in regulation of endothelial nitric-oxide synthase coupling: Relative importance of the de novo biopterin
synthesis versus salvage pathways.
Evaluate the effect of atorvastatin on biopterin
levels and the BH4/BH2 ratio in hypertensive type-2 diabetic patients
Mass Spectrometry of BH4, BH2, and Biopterin
. The optimized electrospray ionization condition should be sensitive enough to detect BH4, DA, IS, BH2, and biopterin
in positive ion detection mode.
Al-Wakeel et al., "Quantitative regulation of intracellular endothelial nitric-oxide synthase (eNOS) coupling by both tetrahydrobiopterin-eNOS stoichiometry and biopterin
redox status insights from cells with TET-regulated GTP cyclohydrolasei expression," Journal of Biological Chemistry, vol.