Biopanning

An affinity selection technique which selects for peptides—e.g., antibody-related peptides—that bind to a given target
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T patents, extending protection to combined use of differential biopanning and high throughput sequencing, such as NGS in identification of antibodies to low expressed cell surface antigens.
In the present study, we sought to identify specific novel peptides or motifs for targeted delivery of drugs or imaging agents by in vivo biopanning method in mammary tumour of female Sprague-Dawley rats induced by LA7 cells.
Alternatively, we propose to modify the Ad capsid or LV envelope using epitopes identified by in vivo biopanning that bind to PR and.
Interesting members from the library can be isolated with simple biopanning selection by binding to an immobilized ligand, and the polypeptide can be identified from its encapsulated DNA [6].
We were interested in examining whether we could obtain peptides having biological activities similar to the GD1[alpha] by biopanning with the phage peptide library and anti-GD1[alpha] MAb.
SP sera are also rich in virus-specific antibodies, which is advantageous for the process of biopanning.
After three rounds of biopanning, the bound phages were identified and amplified, and their DNA was isolated.
T7 is extremely stable in a variety of agents, allowing a wide range of conditions to be employed during the biopanning procedure.
6,030,779 relating to sequence-based gene discovery from the environment, referred to as "genomic biopanning.