Chlorophyll content was determined at 663 and 646 nm and carotenoids were determined at 470 nm using a spectrophotometer (Spectronic BioMate
3 UV-vis Thermo, Madison, WI).
After 15 min incubation in a boiling water bath and then 15-minute incubation in the dark, the absorbance was read at 492 nm on the spectrophotometer (Biomate
3S, UV-Visible Spectrophotometer).
Cells were centrifuged for 10 min., washed with saline solution (0.9%) and standardized in a UV-VIS (Thermo spectronic Biomate
3) spectrophotometer at [600.sub.nm] and D.O = 1 ([10.sup.9] cells [mL.sup.-1]).
The quantitative evaluation was performed by a spectrophotometer (Biomate
3, Thermo Scientific), measuring the UV absorbance at 450 nm.
After this period, the absorbance was measured at 765 nm using a spectrophotometer (Thermo Scientific, UV-Visible Spectrophotometer, Biomate
The absorbance was measured at 490 nm using a spectrophotometer (Biomate
5, Thermo Electron Corporation, Rochester, NY, USA).
D and L also produces BIOmate
, a propriety mixture of additives that makes regular plastics decompose by oxidation and biodegradation.
Centrifuge (Sorvall Evolution RC, USA), ultrasonic disintegrator VibraCell VCX 750 (Sonics, USA), ELISA reader (Bio-Tek, USA), electrophoresis apparatus Mini-Protean III and Semi Dry blotting system (Bio-Rad, USA), Shaker with incubator (ISF-1-W, Kuhner, Switzerland), Spectrophotometer (Biomate
3, Thermo Electron Corporation, U SA), AKTA explorer (GE Healthcare, Sweden) were used in the present study.
Wang et al., "The targeted delivery of anticancer drugs to brain glioma by PEGylated oxidized multiwalled carbon nanotubes modified with angiopep-2," Biomate
Products: Probios food safety interventions; direct fed microbials--BioPlus, Biomate
; yeast--Biomate Yeast Plus; silage inoculants BioSile, Biomax
The Purity and concentration of gDNA were checked using a Biomate
5 spectrophotometer (Thermo Spectronic, USA).