Therefore, in the present study, we isolated the bovine Nanog proximal promoter sequences which presumably contain evolutionarily conserved cis-regulatory sequences, including Oct4/Sox2, NF-[kappa]B, and Sp1 binding sites
, and performed experiments in order to determine their roles in transcriptional activation of the bovine Nanog expression.
Using this method, the heat changes in the titration reaction process can be detected quantitatively and directly and the thermodynamic parameters such as binding constant K, number of binding sites
(N), enthalpy change (H), entropy change (S) and Gibbs free energy (G) can be determined.
10) These data demonstrate that ZE2 and CD81 share similar binding sites
on the P7 fragment of E2, but it needs further investigation
The regulatory machinery works when proteins called transcription factors bind to specific short sequences of DNA that flank the gene, called transcription factor binding sites
, and by doing so, switch genes on and off.
C8[alpha] contains one or more lipid binding sites
that become exposed within the MAC.
Because the actual binding sites
were visualized, he believes that the MOF can be rationally designed to work even better.
The choline ends can occupy multiple choline binding sites
of the CBP simultaneously.
Bioflavonoid interactions with rat uterine type II binding sites
and cell growth inhibition.
More precise identification of the binding sites
is currently being investigated by subjecting labeled peptide fragments to analysis by HPLC and mass spectrometry.
A disadvantage of conventional polymer imprinting is that each "antigen" or template molecule produces an artificial antibody containing all kinds of different binding sites
, but most have poor recognition abilities and are therefore ineffective.
Distinctive hydration patterns for each of the quaternary structures were observed, but a number of conserved water molecule binding sites
were found that are independent of the conformational state of the protein.