Plotting lg[(A0-A)/A] according to lg[Dt] gives the binding constant
K and the binding sites n according to the slope.
The binding constant
for fluoride was calculated from the relative changes in the integrated intensity of NH signals for the free receptor and the complex, yielding a binding constant
larger than [10.sup.4] [M.sup.-1] (Portis et al, 2017).
HBCD-RNA binding constant
was determined as demonstrated previously in UV-Visible spectroscopy (Figure 5A).
CT-DNA study yields intrinsic binding constants
by the arrangement attributing to Hammett's postulation.
Fluorescence anisotropy is widely used to measure the binding constants
and kinetics of reactions that cause a change in the rotational time of the fluorescent molecules .
From the plots the binding constants
(K) for the 1: 1 luteolin/CB7 and EGCG/CB7 complexes were calculated to be 3.2 x [10.sup.3] and 4.8 x [10.sup.3] [M.sup.-1], respectively.
Such surface contact was substantiated by quantitative calculations from (1) for their intrinsic binding constant
([K.sub.b]) and the obtained values are 6.01 x [10.sup.4], 8.16 x [10.sup.5], 5.95 x [10.sup.4], and 7.86 x [10.sup.4] for complexes [C.sub.1], [C.sub.2], [C.sub.3], and [C.sub.4], respectively, which is comparable to a potential intercalator (for EB whose Kb have been found to be in the order of 106-107 M-1) [55, 56].
Furthermore, UV-vis and fluorescence measurements could obtain information related to the binding mechanisms between small molecules and DNA, such as molar ratio, binding modes, binding constants
, quenching rate constants, and thermodynamic parameters .
where [F.sub.0] and F are the fluorescence intensities at 338 nm before and after the addition of the quencher and [L] and [[P.sub.t]] are the ligand and the total protein concentrations, respectively The number of binding sites (n) and the binding constant
([K.sub.b]) were obtained by plotting log(([F.sub.0] - F)/F) versus log(1/([[L.sub.t]] - n([F.sub.0] - F)[[P.sub.t]]/[F.sub.0])) using the reiterative calculation process described by Sun et al.
2b) and the value of the binding constant
(K) for HSA-cisplatin derivative complex is found to be 0.2392 x [10.sup.4] [M.sup.-1].
Flavonoid-serum albumin complexation: Determination of binding constants
and binding sites by fluorescence spectroscopy.
8,921,299 B2; The Procter & Gamble Company has patented a detergent composition comprising tiron (1,2-dihydroxybenzene-3,5-disulfonic acid, or mono- or di-sulfonate salts of the acid); a ligand capable of chelating to Fe3+, wherein the ligand has a binding constant
for Fe3+ that is greater than 1018 M-1, and wherein the ligand is selected from the group consisting of aminocarboxylates containing at least two N atoms, amino-phosphonates containing at least two N atoms, and geminal bisphosphonates; an iron-displacing species selected from the group consisting of phenyl boronic acid, A13+' and mixtures thereof; and at least about 0.2 ppm Fe3+.