(2011) added BglII
and EcoRI restriction sites to forward and reverse primers of Dof1 gene.
The PCR products were digested with BamHI and BglII
and ligated to pTO/IRES2-Venus opened with BamHI.
As done previously for VEEV , WEEV and EEEV DNA vaccine plasmids were then constructed by inserting the synthesized codon-optimized genes into the NotI and BglII
restriction sites of the eukaryotic expression vector pWRG7077 (PowderJect, Madison, WI), which has been described previously .
A 1.7 kb fragment of mouse centromeric minor satellites was amplified from genomic mouse DNA with primers 5-AAA AAA AAG GAT CCA AAA TTT AGA AAT GTC CAC TG-3' and 5-AAA AAA AAA GCT TAA GAT CTC CAT ATT TCA CGT CC-3' and cloned into pBeloBAC 11 into BamH1 and BglII
SLAT2 cDNA was cloned into the BglII
site of pMIG vector, resulting in plasmid pMIG-SLAT2.
To generate luciferase reporter, the sequence of MMP-7 containing MluI and BglII
restriction site was cloned into the upstream of firefly luciferase gene in PGL3 vector.
The pGAPZ[alpha]/gEXNI vector construct was linearized using BglII
The PCR product was cloned to a BglII
site upstream of the flippase recognition target (FRT) in pKD13 (20).
To facilitate cloning into the vector pCAMBIA1301, a restriction enzyme site of PstI was incorporated into a forward primer, whereas a BglII
site was flanked with a reverse primer.
Twenty-one alleles of the IL6 promoter (690 bps, chr7:22732707-22733396) that contained 9 rs1800797 A alleles and 12 rs1800797 G alleles were successfully amplified using primers listed in Supplemental Material, Table S2, from 13 human BEC cultures that were heterozygous for rs1800797 and were directionally cloned into the pGL2-basic luciferase reporter vector (Promega) upstream of the luciferase coding sequence using MluI and BglII
cloning sites according to the manufacturer's instructions.
The amplified fragment was cloned into the NheI and BglII
digested pIRES2-ZsGreen vector for overexpression of WIF-1.
Enzymatic properties and intracellular localization of the novel Trichoderma reesei p-Glucosidase BGLII