Bglii


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Bglii

A type-II homodimeric restriction endonuclease—popularly referred to as bagel two—which cuts DNA in a staggered manner wherever it finds the sequence AGATCT.
References in periodicals archive ?
(2011) added BglII and EcoRI restriction sites to forward and reverse primers of Dof1 gene.
A STRATEGY OF TADOF1 CASSETTE DEVELOPMENT IN PLANT EXPRESSION VECTOR TO ENHANCE NITROGEN ASSIMILATION IN WHEAT
The PCR products were digested with BamHI and BglII and ligated to pTO/IRES2-Venus opened with BamHI.
Importance of the putative furin recognition site [sub.742][RNRR.sub.745] for antiangiogenic Sema3C activity in vitro
As done previously for VEEV [38], WEEV and EEEV DNA vaccine plasmids were then constructed by inserting the synthesized codon-optimized genes into the NotI and BglII restriction sites of the eukaryotic expression vector pWRG7077 (PowderJect, Madison, WI), which has been described previously [40].
A Multiagent Alphavirus DNA Vaccine Delivered by Intramuscular Electroporation Elicits Robust and Durable Virus-Specific Immune Responses in Mice and Rabbits and Completely Protects Mice against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenges
A 1.7 kb fragment of mouse centromeric minor satellites was amplified from genomic mouse DNA with primers 5-AAA AAA AAG GAT CCA AAA TTT AGA AAT GTC CAC TG-3' and 5-AAA AAA AAA GCT TAA GAT CTC CAT ATT TCA CGT CC-3' and cloned into pBeloBAC 11 into BamH1 and BglII sites.
Noncoding Centromeric RNA Expression Impairs Chromosome Stability in Human and Murine Stem Cells
SLAT2 cDNA was cloned into the BglII site of pMIG vector, resulting in plasmid pMIG-SLAT2.
Identification of a Novel Alternatively Spliced Form of Inflammatory Regulator SWAP-70-Like Adapter of T Cells
To generate luciferase reporter, the sequence of MMP-7 containing MluI and BglII restriction site was cloned into the upstream of firefly luciferase gene in PGL3 vector.
Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-[beta]/c-Fos-MMP-7 Pathways
The pGAPZ[alpha]/gEXNI vector construct was linearized using BglII and purified.
Expression, Docking, and Molecular Dynamics of Endo-[beta]-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis
The PCR product was cloned to a BglII site upstream of the flippase recognition target (FRT) in pKD13 (20).
Expansion of Shiga toxin-producing Escherichia coli by use of bovine antibiotic growth promoters
To facilitate cloning into the vector pCAMBIA1301, a restriction enzyme site of PstI was incorporated into a forward primer, whereas a BglII site was flanked with a reverse primer.
Promoter Analysis of Cold-responsive (COR) Gene from Capsella bursa-pastoris and Expression Character in Response to Low Temperature
Twenty-one alleles of the IL6 promoter (690 bps, chr7:22732707-22733396) that contained 9 rs1800797 A alleles and 12 rs1800797 G alleles were successfully amplified using primers listed in Supplemental Material, Table S2, from 13 human BEC cultures that were heterozygous for rs1800797 and were directionally cloned into the pGL2-basic luciferase reporter vector (Promega) upstream of the luciferase coding sequence using MluI and BglII cloning sites according to the manufacturer's instructions.
Radon exposure, IL-6 promoter variants, and lung squamous cell carcinoma in former uranium miners
The amplified fragment was cloned into the NheI and BglII digested pIRES2-ZsGreen vector for overexpression of WIF-1.
Role of Wnt Inhibitory Factor-1 in Inhibition of Bisdemethoxycurcumin Mediated Epithelial-to-Mesenchymal Transition in Highly Metastatic Lung Cancer 95D Cells
Enzymatic properties and intracellular localization of the novel Trichoderma reesei p-Glucosidase BGLII (Ce11A).
Effects of various microorganisms and pretreatments on microbial degradation of lignocellulosic materials
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