Fc receptor expression, concentration of beta-hexosaminidase
, nitric oxide assay, Western blotting, real-time PCR, image analysis, and statistical analysis were measured in cells.
In order to know the evolutional history and properties of plant beta-hexosaminidases, we reconstructed the phylogenetic tree.
beta-Hexosaminidases from each kingdom (plant, animal, and insect) are separated into two clades (clusters) and each clade contains at least one member.
Comparison of serum beta-hexosaminidase
isoenzyme B activity with serum carbohydrate-deficient transferrin and other markers of alcohol abuse.
Immunologic release of beta-hexosaminidase
and beta-glucuronidase from purified rat serosal mast cells.
Increases and time-course variations in beta-hexosaminidase
isoenzyme B and carbohydrate-deficient transferrin in serum from alcoholics are similar.
Both adult and infantile forms of the disease are caused by defects in an enzyme called beta-hexosaminidase
. In the infantile disorder, brain cells do not produce this enzyme, and in the adult case, they produce a poorly functioning enzyme.
Hogikian, "A deletion involving Alu sequences in the beta-hexosaminidase
alpha-chain gene of French Canadians with Tay-Sachs disease," The Journal of Biological Chemistry, vol.
Cells were incubated with vehicle control (saline, final concentration: 0.005%), SC-MN (0.5-1.0 mg/ml, approximately 14-28 [micro]M) (Sigma, prepared for patented use for asthma therapeutic), budesonide ([10.sup.-7] - [10.sup.-6] M), heat-inactivated (50[degrees]C, 3 hr) beta-hexosaminidase
A (HI-Hex A, endogenous ligand for mannose receptor, 50 nM, microdialyzed to remove ammonium sulfate and stored in 5% glycerin-containing phosphate buffer, Sigma), or a combination of SC-MN and HI-Hex A.
found that extracts of Aceriphyllum rossii (native to Korea and China) and its active constituents, quercetin and kaempferol, blocked secretion of beta-hexosaminidase
and histamine; lowered the production and mRNA expression of interleukin-4 and TNF-alpha; and reduced prostaglandin E2 and leukotriene B4 synthesis as well as the expression of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (J.
. Tests for the enzyme beta-hexosaminidase
, which can be detected in the blood, have been used among males to distinguish alcoholics from moderate drinkers and abstainers.