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The different biochemical tests are listed in Table 3: Beta galactosidase, arginine hydrolase, lysine decarboxylase, ornithine decarboxylase test were all negative for all strains.
Lysosomal enzyme testing showed galactocerebroside beta galactosidase or galactocerebrosidase (GALC) level which was almost undetectable and definitely much below the 99th percentile for age and sex confirming the diagnosis of Krabbe disease.
On determining the activity of this group of enzyme, they found that Beta galactosidase, G N acetyl galactosaminidase and [beta] -N acetyl glucosaminidase were the most active.