Ber-Ep4

Ber-Ep4

A monoclonal antibody used in histopathology to differentiate glandular epithelium (usually positive) from mesothelium (usually negative).
References in periodicals archive ?
2 and Ber-EP4 are generally considered to support the diagnosis of EMPD;[3] however, some cases of pagetoid BD show positive for them.
48,76-79) Unlike BCC, TFI displays colonizing CK20+ Merkel cells, and lacks Ber-EP4 staining.
No immunoreaction was observed in the tumour tissue with pankeratin, epithelial membrane antigen (EMA), Ber-Ep4, smooth muscle actin (SMA), desmin, CD117, CD34, bcl-2, CD10, and S100 protein.
Systematic reviews of 17 studies have reported sensitivities and specificities of Ber-EP4 in pulmonary adenocarcinoma as 80% and 90% respectively8.
Results were negative for carcinoembrionary antigen and the epithelial glycoprotein Ber-EP4, whereas results were positive for the mesothelial markers cytokeratins, calretinin, epithelial membrane antigen, and HBME-1, thus leading to the correct diagnosis of peritoneal epithelial mesothelioma.
Efficiency of Ber-EP4 antibody for isolating circulating epithelial cells before RT-PCR detection.
1) The utility of Ber-EP4 in this setting is controversial; although BCCs stain positive for Ber-EP4, there is varying literature on its reactivity in sebaceous lesions.
Thymic papillary carcinoma shows variable positivity for CD15, Ber-EP4, and carcinoembryonic antigen.
Immunohistochemical studies were performed on 5-[micro]m-thick sections of formalin-fixed, paraffin-embedded tissue, using standard techniques, heat-induced epitope retrieval buffer, and primary antibodies against BerEP4 (clone Ber-EP4, 1:20; Dako, Carpinteria, California), CK14 (clone LL002; Cell Marque, Rocklin, California), and CK17 (clone E3, 1:50; Dako).
1) Based on their sensitivity and specificity, calretinin (Figure 11, A and B), CK5 or CK5/6 (Figure 12, A and B), WT-1 (Figure 13, A through C), and D2-40 (podoplanin) (Figure 14, A and B) are the best positive mesothelioma markers; and MOC-31 (Figure 15, A through C), Ber-EP4, carcinoembryonic antigen (CEA), and Lewis(y) antigen blood group 8 (BG8) are the best overall carcinoma markers.
Value of the Ber-EP4 antibody in differentiating epithelial pleural mesothelioma from adenocarcinoma: the M.
2, CK18, and CK7 are positive more frequently than epithelial membrane antigen, carcinoembryonic antigen (CEA), CD15, and Ber-EP4.