Immunohistochemistry staining showed positive Ber-EP4
[Figure 3]b and Bcl-2 [Figure 3]c.
(1 ) PA is positive for CK19 and negative for Ber-EP4
, whereas the opposite is true for BCC.
Immunohistochemistry is not useful but numerous stains are given in the literature which include Bcl-2, Ber-Ep4
and CD 34.
Immunohistochemical stains for high-molecular-weight cytokeratin and Ber-EP4
highlighted the basal cell carcinoma.
In this study, we report and discuss the results of 17 immunohistochemistry markers used in the differential Immunohistochemistry in Peritoneal Mesothelioma--Tandon et al diagnosis of mesothelioma--anti-TAG72 antibody (B72.3), breast cancer 1-associated protein (BAP1), anti-epithelial cell adhesion molecule antibody (Ber-EP4
), calretinin, syndecan1 (CD138), carcinoembryonic antigen (CEA), cytokeratin 20 (CK20), cytokeratin 5/6 (CK5/6), cytokeratin 7 (CK7), antioncofetal protein M2A antibody (D2-40), estrogen receptor (ER), Lewis X antibody (CD15), mesothelin, tumor protein 63 (p63), paired box gene 8 (PAX8), progesterone receptor (PR), and Wilms tumor protein (WT1)--in a large series of peritoneal mesothelioma cases.
Barut, "The use of Ber-EP4
antigen in the differential diagnosis of basosquamous carcinoma from squamous and basal cell carcinoma," Turk Patoloji Dergisi, vol.
antibody as a discriminant in the differential diagnosis of malignant mesothelioma versus adenocarcinoma," American Journal of Surgical Pathology, vol.
Marras et al., "Immunohistochemical evaluation of epithelial antigen Ber-Ep4
and CD10: new markers for endometriosis?" European Journal of Gynaecological Oncology, vol.
CAM5.2 and Ber-EP4
are generally considered to support the diagnosis of EMPD; however, some cases of pagetoid BD show positive for them. Recent studies suggest that p63 might be a useful marker as it stains atypical keratinocytes of BD but not EMPDs. This patient's tissue [Supplementary Table 1] [SUPPORTING:1] shows positive for P63, Ki-67 (<30%), and CK34[sz]E12, weakly positive for CAM5.2, and negative for PAS, S100, HMB-45, CEA, and GCDFP15.
No immunoreaction was observed in the tumour tissue with pankeratin, epithelial membrane antigen (EMA), Ber-Ep4
, smooth muscle actin (SMA), desmin, CD117, CD34, bcl-2, CD10, and S100 protein.
Systematic reviews of 17 studies have reported sensitivities and specificities of Ber-EP4
in pulmonary adenocarcinoma as 80% and 90% respectively8.
Results were negative for carcinoembrionary antigen and the epithelial glycoprotein Ber-EP4
, whereas results were positive for the mesothelial markers cytokeratins, calretinin, epithelial membrane antigen, and HBME-1, thus leading to the correct diagnosis of peritoneal epithelial mesothelioma.