The determination of cocaine, benzoylecgonine
, and cocaethylene in small-volume oral fluid samples by liquid chromatography-quadrupole-time-of-flight mass spectrometry.
Two or three technologists were needed each day to perform GC/MS data analysis for six assays: alkaline and acid scans, opiates, benzoylecgonine
, benzodiazepines, and cannabinoids.
The BHA confirmed he also gave a urine sample at Southwell in March that tested positive for benzoylecgonine
Both players' urine tests returned findings for benzoylecgonine
, the major metabolite of cocaine, after they were selected for an out-of competition test between April and June this year.
Using selected reaction monitoring (SRM) and isotope dilution, we validated the use of this system for the quantitative analysis of benzoylecgonine
Then we conducted a literature search that revealed data indicating the temporal excretion pattern of ingested cocaine.|2-4~ Everything fit: The amount of benzoylecgonine
(BE), the major metabolite of cocaine, was compatible with the amount that should have been in the urine 10 hours after drinking one cup of mate de coca.
After being arrested, Higgins was found to have 253 micrograms of benzoylecgonine
- the main metabolite of cocaine - per litre of blood.
Mr Byrne had 635mg of cocaine in his body, and Wirral coroner Andre Rebello said the "relatively high" levels of benzoylecgonine
, the main metabolite of the drug, "could suggest binge use or caused by previous excessive use".
The jockey has not ridden since the end of September when his licence was suspended after he gave a urine sample at a Lingfield race meeting that tested positive for benzoylecgonine
, a metabolite of cocaine.
Marrow was 13 times over the limit for Benzoylecgonine
, the metabolised form of cocaine, four times over the cocaine limit and double the cannabis limit.
The 21-year-old was found guilty of having Benzoylecgonine
, a metabolite of cocaine, in his system after providing a urine sample.
Also a Class A substance, Benzoylecgonine
, a diagnostic metabolite of Cocaine, was shown to be present.