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In the case of two absorbing compounds, the concentrations of each can be calculated from measurements of light attenuation at two different wavelengths using the Beer Lambert law - the same principle used in pulse oximetry.
In the second phase, the resulted colored solution is photometrically analyzed at a wavelength corresponding to followed species with the aim of establishing the optical absorbance of the solution and absorbance value is correlated with concentration through the Beer Lambert Law (Matter, 1995, Skoog, 1996, Robinson, 2005).