Beer-Lambert law

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Beer-Lam·bert law

(bēr lam'bert),
the absorbance of light is directly proportional to the thickness of the media through which the light is being transmitted multiplied by the concentration of absorbing chromophore; that is, A = εbc where A is the absorbance, ε is the molar extinction coefficient, b is the thickness of the solution, and c is the concentration.
[August Beer, Johann Heinrich Lambert]
Farlex Partner Medical Dictionary © Farlex 2012

Beer’s law

A law stating that the concentration of an analyte is directly proportional to the amount of light absorbed, or inversely proportional to the logarithm of the transmitted light.

Beer’s law
A = abc = log(100/%T) 2 - log %T

A = absorbance
a = absorptivity
b = light path of the solution in cm
c = concentration of the substance of interest
%T = per cent transmittance—the ratio of transmitted light to incident light
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.


August, German physicist, 1825-1863.
Beer-Lambert law - the absorbance of light is directly proportional to the thickness of the ligand through which the light is being transmitted multiplied by the concentration of absorbing chromophore.
Beer law - the intensity of a color or of a light ray is inversely proportional to the depth of liquid through which it is transmitted.


Johann Heinrich, German mathematician and physicist, 1728-1777.
Beer-Lambert law - see under Beer, August
Lambert cosine law - mathematical measure of the intensity of radiation.
Medical Eponyms © Farlex 2012
References in periodicals archive ?
Estimation of net radiation absorbed by leaf area unit of coffee plants using Beer's law, integrated in periods of 15 min, resulted in values which agreed well with the measurements by the moving system, with a tendency of overestimation of about 10% (Figure 7A), indicating the possibility to estimate Rnc from the measurement of global radiation or gross net radiation, leaf area index and extinction coefficient.
The Beer's law relationship between T and LAI, and the extinction coefficient k, were determined (Eq.
Using the Omnic software and TQ Analyst program, calibration models based on simple Beer's law and partial least square (PLS) were optimized for the quantitative determination of total phenolic content in lemon samples.
The adherence to Beer's law was studied by measuring the absorbance value of the series of solutions containing different concentrations of the metal ion.
However, many of the pigments used in automotive coatings do not function by absorption where Beer's law has been shown to apply.
The stability of absorbance value in dissolution medium was crossed checked against the absorbance value of freshly papered solution and the relation of absorbance with concentration was determined according to beer's law. Due to sensitive, stable and repeatable absorbance values of SOF at 262 nm, wavelength 262 nm was selected for quantitative determination.
The Beer's law limits and molar absorptivity values were evaluated and given in Table1 which indicated that the method is sensitive.
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In order to investigate the range in which the colored complex adhere to Beer's law, the absorbance of the complex was measured at emax for a series of solutions containing increasing amounts of PEH drug (Fig.
The color reaction obeys Beer's law from 0.1 to 2 mg/10 mL of diazepam.
For the development of calibration simple Beer's law and PLS (Partial least squares) both were tested with different measurement criteria like peak area, peak height using baseline correction to evaluate calculation of the analyte for recorded FT-IR spectra of clarithromycin standards ranging from 0.005 to 2.0 mg.