By using GAPDH as the reference gene, the mRNA altitude of Bcl-2 genes
was resolved through (DCt) method, through the deduction of average DCt of the sample from the DCt of the control the DDCt values were calculated and were converted to fold difference by raising 2 to the power DDCt (2^DDCt).
In addition, investigators have shown that Bcl-2 gene
family is known to modulate the permeability of the mitochondrial membrane and the release of cytochrome C .
promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells.
The study findings potentially could refine this approach so that chemotherapy can be timed more effectively, killing off cancer cells and preventing a relapse when the BCL-2 gene
is sufficiently weak.
Involvement of the bcl-2 gene
in human follicular lymphoma, Science, 228: 1440-3.
High-mobility group A1 proteins regulate p53-mediated transcription of Bcl-2 gene
Differential expression of members of the bcl-2 gene
family in proliferative and secretory human endometrium: glandular epithelial cell apoptosis is associated with increased expression of bax.
Importantly, the Trinity team also discovered that members of the Bcl-2 gene
family could override this process, switching off the self-eating process and leading to survival of cancerous cells.
These results can be explained on the basis of findings of Chattopadhya et al , which showed that I/R injury inhibits Bcl-2 gene
expression resulting and bind to the "permeability transition pore" of mitochondrial membrane inducing loss of selective ion permeability which results in release into the cytosol intermembrane space contents including cytochrome c and apoptosis-inducing factor (AIF).
HL-60bcl-2 cells, which are derived from a stable transfectant clone of the human bcl-2 gene
into the HL-60 leukemia cell line, show 5-fold greater expression of the Bcl-2 protein compared with HL-60neo cells, a control clone transfected with vector alone.
protein expression in salivary gland tumors.
The patient's positive BCL-2 gene
findings helped to clinch the diagnosis.