These SNPs were harboured in genes known to play a critical role in bone formation and remodeling, including the MALAT1, MRPL39, FASLG, FAM189A2, RP11-15A1.7, LSAMP, and BMPR1B
The BMP receptors are a family of transmembrane serine/threonine kinases that include the type I receptors Bmpr1a and Bmpr1b
, and the type II receptor Bmpr2 . Deletion of the receptor Bmpr1a in the osteoblast lineage cells with a Dmp1 -Cre caused a dramatic increase in trabecular bone mass in postnatal mice, which was due to a marked increase in osteoblast numbers.
Using this procedure, we found similar gene expression profiles for INHA, IGFBP5, BMPR1B
, BMP2, INSIG, PTGS2, LOC102181730 and LOC102184274, which indicated the high credibility of these genes in transcript abundance; however, some factors in the RNA-seq results, including BAX, LOC102183322, and BCL2, were inconsistent (Fig.
Genotyping of BMPR1B
, BMP15 and GDF9 genes in Chilean sheep breeds and association with prolificacy.
Using real time quantitative PCR (qPCR), we observed significant upregulation of BMP2, BMPr1b
, Tuft1, OPG, and Wnt10b upon IL-27 treatment in differentiating osteoblasts/osteocytes (*P < 0.05; Figure 2(a), right plot).
Bone morphogenetic protein receptor, type IB (BMPR1B
) encodes a member of the bone morphogenetic protein receptor family of transmembrane serine/threonine kinases, which are involved in endochondral bone formation and embryogenesis.
Overexpression of a dominant-negative BMPR1B
also inhibited osteoblast differentiation in osteoblast precursor cells (25).
Ahmed et al., "The prevalence of MADH4 and BMPR1A mutations in juvenile polyposis and absence of BMPR2, BMPR1B
, and ACVR1 mutations," Journal of Medical Genetics, vol.
Expression of TGF[beta]1, VEGFA, COL1, POSTN, RUNX2, Ki67, TGF[beta] receptors (TGF[beta]R1, TGF[beta]R2), BMP receptors (BMPR1A, BMPR1B
, and BMPR2), leptin, full-length LEPR, IL-6, tumor necrosis factor (TNF) [alpha], cyclooxygenase (COX) 2, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected by real-time PCR using the iCycler iQ detection system (Bio-Rad Laboratories), SYBR Green (Bio-Rad Laboratories), and specific primers (QuantiTect Primer Assay, Qiagen).
Components identified from the arrayed clones of the EST collection include bmp2/4, bmp5-8, twg, cv-2, xolloid/tolloid, chordin, noggin, follistatin, gremlin1, gremlin2, neuralin, dan, kielin-like, bmpR1B
, bmpR2, bambi, smad1/5, smad4, smad6/7, smurf, and tak.