These include the ability to reduce intratumor BMI1
associated with elimination of the stem cell-like phenotypes, and the lack of any detectable toxicity.
Yin Yang 1 is associated with cancer stem cell transcription factors (SOX2, OCT4, BMI1
) and clinical implication.
As a member of PRC1, B-lymphoma Mo-MLV insertion region 1 (BMI1
) has been reported to be overexpressed in advanced stages and related to poor prognosis in many cancers (19).
This project focuses specifically on understanding the role of BMI1
, a stem cell protein that promotes tumor growth when present in high levels within the cell.
 In addition to regulating various cellular processes, miRNAs are epigenetic modulators by targeting mRNAs of epigenetic regulators including DNA methyltransferase 3 alpha (DNMT3A), DNA methyltransferase 3 Beta (DNMT3B), polycomb mRNAs, EZH2 (as shown above), BMI1
Relative quantification of the mRNA level of Bmi1
was determined using the 2−??Ct method. Each experiment was repeated for three times in duplicates.
Qi et al., "Bmi1
regulates the proliferation of cochlear supporting cells via the canonical Wnt signaling pathway," Molecular Neurobiology, vol.
Wang et al., "Bmi1
is essential in Twist1-induced epithelial-mesenchymal transition," Nature Cell Biology, vol.
They also demonstrated that tumors from Villin-Lin28b/let-7IEC-KO mice exhibited a significant upregulation of stem cell markers including Bmi1
, Lrig1, Olfm4, ASCL2, Prom1, LGR5, Msi1, and SOX9, suggesting an expansion of CRC and +4 stem cell-like compartments.
was identified as a barrier to reprogramming by modifying histone marks at key cardiogenic loci, thus inhibiting iCM induction.
Bcl2, Ccnd1, Cdk6, and Sox4), intestinal stem cells (Lgr5, Olfm4, and Bmi1
) , mucin biology (Muc2 and Muc6), and tight junctions (occludin, zonulin-1, and Jam) was analyzed using real-time PCR (Table S1).