BKV


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BKV

BK virus.
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BKV DNA polymerase chain reaction (PCR) (QIAGEN, Hilden, Germany) was performed every month in the first six months, then every three months and/or when creatinine elevation was detected.
Detecting BKV and JCV with PCR is of high sensitivity and specificity and is helpful in timely discovering and treating polyomavirus infection.
Seroepidemiologic data for BKV, JCV, and MCPyV indicate that human polyomavirus infections are ubiquitous and generally occur without apparent disease.
4; EraGen Biosciences, Madison, Wisconsin) and a reagent kit, including 2 x PCR reaction buffer and 25 x primer mix (including primers for both BKV and internal amplification control).
Semi-nested PCR was performed in order to detect the BKV DNA, as validated by Nickeleit et al.
Polyomavirus-positive urine samples were subjected to seminested PCR to differentiate BKV and JCV.
N-terminal-tagged GST-VP1s from BKV, JCV, and simian virus 40 (SV40) were generously provided by Michael Pawlita (16), and GST-tagged microneme (Mic) protein encoded by Toxoplasma gondii was provided by David Sibley.
14) A few studies (15-20) have considered the possibility that specific BKV TCR sequence variations are related to the development of PVAN, but the results have, so far, been discordant, and their real significance in human hosts has not yet been defined.
However, the specificity of the SV40 enzyme immunoassay is problematic because studies with serum samples from macaques have found that antibodies are cross-reactive with polyomaviruses JCV and BKV (4).