culture is a known substrate produced for the rabies in animals.
The significant differences noted regarding the cytotoxicity of the synthesized compounds in both the experiment and control cells were supported by the ratio of the concentration that caused half of the BHK cell
line to die to the concentration that caused a similar proportion of death in HCT-116 [50-52].
As shown in Figure 5(a), wtSOD1 transfection remarkably inhibited whereas mhSOD1/C111S has no apparent effect on BHK cell
expression showed that the transporter was located in the cell membrane.
Specimens available in sufficient quantity were inoculated into RD and BHK cell
The results demonstrated that the SHZH05 strain and the CC024, CC045, CC090, and CC163 strains induced similar CPEs in Vero and BHK cells
at 96 h post-infection (Figure 1).
In addition, multiple attempts to isolate this virus on various cell lines of eukaryotes (Vero E6, MDCK, CRFK, N2a, and BHK cells
, primary fox kidney cells) failed because of the absence of cytopathic effects and viral replication by quantitative reverse transcription PCR, despite a high number of reads in the original sample.
Adaptation of Sindbis virus to BHK cells
selects for use of heparan sulfate as an attachment receptor.
Recent data show that Sindbis virus can penetrate mosquito C7-10 cells even more quickly than what has been seen in BHK cells
. By 60 min.
The immunofluorescence test was performed using SBV-infected BHK cells
, clone BRS5 (L194, Collection of Cell Lines in Veterinary Medicine, Greifswald-Insel Riems) as antigen matrix in accordance with the procedure described previously .
We therefore submitted selected samples (11 whole blood samples, 4 serum samples, and 15 supernatant samples from the BHK cells
) to the World Organisation for Animal Health reference laboratory for BTV (Institute for Animal Health, Pirbright, UK) for virologic and serologic analysis.