16S rRNA PCR, 23S-5S rRNA PCR, sequencing of the mip-gene, and cultures of these samples on BCYE agar
allowed the identification of L.
The remaining sample in the suspension was heat-treated for 30 minutes at 50[degrees]C, and was inoculated to BCYE agar supplemented with GVPN.
For the isolation and counting of Legionella bacteria, 0.1 mL of the homogenized biofilm suspension was inoculated into BCYE agar supplemented with GVPN directly and after treatment with acid and heat as described above.
Colonies growing on BCYE agar, but not on blood agar, were definitively identified as Legionella spp.
pneumophila isolates were cultured on BCYE agar, and their growth was confirmed using a serotyping kit.
BCYE agar plates were incubated in an extinguished candle jar at 37 [degrees]C for 72 h.
The sample was filtered through 0.45 [micro]m membrane filters, the filter was placed on BCYE agar, and incubated in a candle jar at 37 [degrees]C for 72 h.
Culture: All respiratory fluids were cultured on selective BCYE agar. Before inoculation, all sputum samples were treated by simple dilution method (11).
pneumophila (ATCC 331522) is maintained on BCYE agar and used as a control for culture of clinical specimens.
Four days later, Legionella species were isolated on BCYE agar
(Oxoid Ltd.) and confirmed by DFA (Legionella Poly-ID Test Kit, Remel, Lenexa, KS, USA) and also by L.
Since Legionella is spread by aerosolized water, the organism can be recovered from the air environment by concentrating it with the N-6 impactor on culture media composed of buffered-charcoal yeast extract (BCYE) agar and BCYE agar
plus polymyxin B, anisomycin, and vancomycin (BCYE/PAV).