For example, the isolated eye tests IRE and CEET as well as the BCOP and HET-CAM tests are accepted by some European regulatory authorities on a case-by-case basis for the identification of severe eye irritants for the purposes of classification and labeling within the European Union on chemicals and products.
BCOP chief executive Marcus Fellows said it would not have achieved its success without the help and support of numerous individuals and organisations, "including its own employees, volunteers, supporters, close working suppliers, contractors and professionals who have helped us over the last 70 years."
TABLE 1: ENDPOINTS AND OECD-VALIDATED ALTERNATIVE IN VITRO TEST METHODS ITS: Integrated testing strategy Toxicological Endpoint Test Method Test method description Ocular irritation BCOP Opacity and permeability to fluorescein measurement ICE Swelling of the cornea, opacity, fluorescein retention, morphology Fluorescein leakage Measurement of test fluorescein leakage trough a mono-layer of confluent cells MDCK CB997 Het-Cam Observation of the Irritating effects (hyperaemia, haemorrhages, coagulation) EpiOccular, HCE Human corneal epithelium SkinEthic model.
Four of them, the Isolated Chicken Eye test (ICE), the Bovine Corneal Opacity and Permeability assay (BCOP), the Short Time Exposure test (STE) and the Reconstructed human Cornea-like Epithelium test (RhCE) can be used to identify chemicals that do not require GHS classification (No Cat.) or that induce serious eye irritation (GHS Cat.
The nonanimal methods used were: the bovine corneal opacity and permeability (BCOP) assay (Gautheron et al., 1994), the Cytosensor[TM] microphysiometer (CM) assay (Hartung et al., 2010), and the EpiOcular[TM] (EO) time to toxicity assay (MatTek Corporation, Ashland, MA) (Stern et al., 1998).