1.5 x [10.sup.5] PBMC/well from 8 pigs before infection (0 dpi) and day 28 post infection (28 dpi) were stimulated in triplicate with recombinant GRA1, GRA2, GRA7, GRA9, BAG1
, or SAG1 (1 [micro]g/ml) and T cell proliferation was determined via [sup.3]H- thymidine incorporation.
The quadratic and cubic polynomial terms were nonsignificant (P >0.05) for 6 genes (ACTB, BAG1
, CD68, ESR1, RPLPO, and TFRC), indicating linear performance over the entire RNA range tested (2-1[degrees] to 8 ng).
In addition, the disease-related mutation DJ-1 L166P shifts its subcellular distribution to the nucleus and decreases its ability to dimerize, whereas BAG1
, the first discovered BAG family protein, restores DJ-1 L166P subcellular distribution and dimerization .
Chen, "Mechanism of BAG1
repair on Parkinson's disease-linked DJ1 mutation," Journal of Biomolecular Structure and Dynamics, vol.
Consistent with these observations, [E.sub.2] also induces the apoptotic regulators BCL2 and BAG1
in cultured breast cancer cells (Perillo et al.