As seen from Table 6, antimicrobial activity was only displayed by the hexane leaf extracts toward B. subtilis
(10 mm) and S.
These results showed that B. subtilis
promotes positive influence on plant growth under normal conditions (without salinity).
Extracted pHT43-TPD was used for transformation of B. subtilis
(WB600) by Eppendorf Eporator (Eppendorf, USA) according to the B.
For B. subtilis
BML5 identification, morphological and various biochemical tests such as Gram staining, catalase test, oxidase test, citrate utilization, lactose fermentation, mannitol test, starch hydrolysis, methyl red test, indole test and Voges-Proskauer test were performed.
To meet the requirement in swine production, we used B. subtilis
to construct a derived strain engineered to express AMPs of pBD-2 and cecropin P1, and then analyzed its antimicrobial activity and growth-promoting effects in vitro and in vivo.
 showing that the Landy medium which contains glutamic acid as nitrogen source promotes the synthesis of lipopeptides by B. subtilis
. Other nitrogen sources have been also reported to support lipopeptides production by some Bacillus strains like urea  and ammonium nitrate .
In addition, the selected antagonist, B. subtilis
BAS114, was studied for its ability to antagonize the growth of pathogenic fungi and a bacterium causing the major rice diseases, dirty panicle disease, sheath blight disease and leaf blight disease, and Phomopsis blight in asparagus.
In this study, a strain of B. subtilis
which had been isolated from soil of pistachio orchards was grown in different culture conditions including different incubation times, temperatures and culture media.
Chicken feather was also previously revealed to be an efficient substrate for keratinase production by B. subtilis
G8 (Cheepudom, 2010).