Cytoplasmic projections with tongue-like protrusions and fine or coarse cytoplasmic azurophilic granules
were also present (Figure 3).
As shown in Figure 5(c), both SP and CGRP did not inhibit the OMV-induced neutrophils to release the azurophilic granules
. In contrast, the release of specific granules was significantly decreased in the presence of CGRP (Figure 5(d)).
Diagnosis can be made with a simple peripheral smear for the classic giant azurophilic granules
, which are peroxidase-positive, in all granule-containing cells including the peripheral blood and bone marrow.
The specific granules are smaller with 0.1 [micro]m diameter and formed after azurophilic granules
. These granules do not contain MPO and are characterized by the presence of the glycoprotein lactoferrin.
The enzyme [alpha.sub.1] antitrypsin is a serine protease inhibitor capable of neutralizing the proteolysis caused by the neutrophil elastase released from the azurophilic granules
of these cells .
In humans, azurophilic granules
can be differentiated into defensin-high and defensin-low .
In positive cases, 2 main fluorescent patterns will be detected in ethanol-fixed neutrophils: one that reacts with the azurophilic granules
distributed throughout the cytoplasm (cytoplasmic pattern, called cANCA) and one that reacts only with antigens located in the cytoplasm surrounding the nucleus (perinuclear pattern, called pANCA).
In contrast with Santos (2001) for carijo hawk (Buteo magnirostris), in this study, two azurophilic granules
were not observed in one of the thrombocyte poles in rhea.
Identification of cell abnormalities--such as the large azurophilic granules
in toxic granulation, asynchronous maturation, micromegakaryocyte identification or quantitating variant lymphs--still requires a skilled human eye.
To the Editor: Large granular lymphocytic leukemia (LGLL) is a rare chronic clonal lymphoproliferative disease characterized by a persistent increase in large granular lymphocytes, which are large lymphocytes with a low nucleus to cytoplasm ratio and an abundant cytoplasm with azurophilic granules
. [sup] Here we report two cases diagnosed as refractory LGLL treated with porcine antihuman thymocyte immunoglobulin (ATG) in West China Hospital.
Electron microscopic studies suggest that these granules are formed by the fusion of azurophilic granules
(1,2) The appearance of TG is the result of an increase in acid mucosubstance in azurophilic granules
, which stains more prominently than under normal circumstances.