cleistogamy

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cleistogamy

the production of small, inconspicuous flowers in which self-fertilization occurs before the flowers open. For example, the violet forms large, showy, cross-pollinating flowers, but also forms cleistogamic flowers late in the summer.
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The automatic self-pollination can hinder cross-pollination pollen tube growth, owing to competition with the autogamic pollen tubes, thus reducing xenogamy rate (MAHORO, 2003; SILVA; PINHEIRO, 2009).
Natural biotic pollination did not significantly increase the amount of pollen grains on the stigma, and therefore automatic self-pollination is possible in this species.
Self-pollen grains from automatic self-pollination were dislodged from the stigmas into solution by sonication, stained with toluidine blue, filtered by vacuum filtration onto paper, and counted under a light microscope.

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