Aspartate aminotransferase (AST), alanine aminotransferase (ALT), TBIL, albumin (ALB), total bile acid (TBA), gamma-glutamyl transpeptidase (GGT) and alkaline phosphatase (ALP) were quantitated using an autoanalyser
(SYSMEX, Tokyo, Japan).
Alkaline Phosphatase was determined on autoanalyser
(Erbamannhein chemistry autoanalyser
, Germany) using standard Erba kits according to IFCC recommendation utilizing 4-nitrophenyl phosphate as the substrate 20.
Liver enzymes: AlanineAmino Transferase (ALT), Aspartate Amino Transferase (AST), Gamma-Glutamyl-Transferase (GGT) and Alkaline Phosphatase (ALP) were measured by Vitros 350 autoanalyser
(New York, USA; Product code 680-2153) using their accompanying commercial kits (Vitros Chemistry Products, Ortho-Clinical Diagnostics, Johnson-Johnson Company, New York, USA).
Serum protein analyses were performed with commercial kit (Audit, Irland) via autoanalyser
(Autolab, The Netherlands).
bil), totalprotein (TP), gamma glutamyltransferase (GGT), blood urea nitrogen (BUN) concentration measurements were performed in autoanalyser
(Roche Cobas C111 Germany) by using the Roche Diagnostics Germany commercial kit.
Other biochemical tests were all performed using routine clinical autoanalyser
assays in the Biochemistry Department of Zhongshan Hospital, including NT-proBNP, serum cholesterol, triglycerides, alanine aminotransferase, urea nitrogen, and creatinine.
The water soluble carbohydrate (WSC) content was determined using an autoanalyser
according to the method used by Fulkerson et al.
Serum uric acid and creatinine were estimated immediately on Dade Behring Dimension Xpand plus autoanalyser
The subjects underwent the following tests: blood pressure examination to rule out hypertension, complete blood count and platelet parameters such as platelet count (PLT), plateletcrit (PCT), MPV, PDW, and platelet-large cell ratio (P-LCR) were estimated using Sysmex II Autoanalyser
The concentration of hs-CRP was measured using the immunoturbidimetric method by Abbott Architect C16000 autoanalyser
(Abbott Diagnostic, USA).
The obtained samples were immediately centrifuged and their respective lipid levels were determined by applying enzymatic procedure using German Rosh kits, with Biochemical Autoanalyser
Prestige 24i (Japan).
Fasting blood glucose was done by fully automated autoanalyser
(CX-4 analyser by Beckman Coulter USA).