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An instrument capable of conducting analyses automatically; commonly used in chemical analyses.
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The WEOM extracts were analysed for pH, EC, total OC (TOC, TOC-V CSN autoanalyser, Shimadzu Corporation, Kyoto, Japan), total dissolved N (TDN, TNM-1 autoanalyser, Shimadzu Corporation), nitrate content (ion chromatography), [E.sub.4]/[E.sub.6] ratio, as well as fluorescence and Fourier transform infrared (FT-IR) spectra.
At the end of the experiment, following intraperitoneal anaesthesia with thiopental sodium 50 mg/kg, blood samples (3 ml) were taken from the left ventricle for the measurement of total serum levels of sodium, chloride, potassium, calcium, and magnesium using a standard autoanalyser technique (Architect c16000 Autoanalyzer, Abbott Diagnostics, MA, USA) and total serum immunoglobulin E using ELISA.
Fresh serum samples were analyzed to determine of serum Ca, P and Mg levels by autoanalyser (Model: Gesan-Chem200, No: 1102422, Campobello-Italy) using its commercial kit (Monore-agent-LR-C2230150V, Italy).
Spot urine sample from each subject was quantified for micro albumin by using solid phase sandwich ELISA on Dia 710 micro plate reader (Made in Australia)12 and creatinine by modified Jaffe method on chemistry autoanalyser (Erbamannhein Germany)13.
Blood urea nitrogen and blood creatinine levels were analysed photometrically with a Beckman AU 5800 autoanalyser. Serum NGAL levels were analysed with the enzyme-linked immunosorbent assay, using a Boster trade kit (Boster Biological Technology Co., CA; cat number: EK0855, USA).
Plasma samples, collected from 37 patients, and 53 controls, were dissolved and the plasma BNP measurements were performed by the chemiluminescence microparticle immunoassay method by an Abbott architect ci4100 biochemistry autoanalyser using a 100-well Abbot Architect BNP Reagent Kit.
Nitrogen value was characterized using the Kjeldahl procedure (Kjeltec 2300 Autoanalyser, Foss Tecator AB, Hoganas, Sweden) and CP was estimated as Nx6.25.
Aspartate aminotransferase (AST), alanine aminotransferase (ALT), TBIL, albumin (ALB), total bile acid (TBA), gamma-glutamyl transpeptidase (GGT) and alkaline phosphatase (ALP) were quantitated using an autoanalyser (SYSMEX, Tokyo, Japan).
Serum urea, creatinine, sodium ([Na.sup.+]), potassium ([K.sup.+]) levels; alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatine kinase (CK) activities; and urine creatinine and sodium measurements were performed on an autoanalyser (Konelab Prime 60i, Thermo Scientific, Finland) in Trakya University Biochemistry Laboratory.
Fasting plasma glucose (FPG), 2-hour postglucose load plasma glucose (2 h PPG), total cholesterol, high-density lipoprotein cholesterol (HDL), triglyceride, and creatinine were analysed using BS 400 chemical autoanalyser (Mindray, China) and commercial reagents (Randox Laboratory Reagents, UK).
FPG, 2h-PPG, total cholesterol, high-density lipoprotein cholesterol (HDL), and triglyceride levels were analysed by colorimetric enzymatic assays using BS 120 chemical autoanalyser (Mindray, China) and commercial reagents (Randox Laboratory Reagents, UK).