com); if Indian ink positive and HIV suspected, culture on Sabouraud agar Mycobacterium Lowenstein-Jensen None tuberculosis culture; auramine
and Ziehl-Neelsen stains Rickettsia spp.
Bright field microscopy uses carbol fuschin method which involves Ziehl-Neelsen technique and Fluorescence Microscopy comprises fluorochrome procedure using Auramine
O or auramine-rhodamine stain.
It can be also be performed using Ziehl-Neelsen (ZN) staining or fluorescence microscopy (FM) using a fluorescent substance such as auramine
phenol to increase the visualization (Ogbaini-Emovon, 2009)
Then, all the smears were stained with auramine
O and examined with fluorescence microscopy for acid fast bacteria (AFB).
It shows that using IS6110 as a target gene in molecular pathology technique to diagnose TB patients has higher positive rate (51.5%), when compared with acid fast stain (31%) and auramine
O fluorescent dyes (40.5%) with P < 0.05, and could distinguish non-TB Mycobacterium from MTB.[sup] In China, molecular biology technique confines to sputum samples, and traditionally, pathology diagnosis confines to histomorphology and pathogen investigation, both of which may have low sensitivity and specificity.[sup] Molecular pathology may be a better choice, especially for rare disease diagnosis, where diagnosis often depends on pathology.
Sputum samples were screened by fluorescent microscopy using Auramine
O stain and smears found to be positive were confirmed by light microscopy using Ziehl-Neelsen's stain as per the standard protocols of both staining methods.
rhodamine dye is then used to stain the cells and visualized using a fluorescence microscope .
Samples collected in 2016 (n = 282) were stained using auramine
O staining and read with a fluorescent light-emitting diode (LED) microscope (40x).
Selvaganapathi, "Constrain in solving Langmuir-Hinshelwood kinetic expression for the photocatalytic degradation of Auramine
O aqueous solutions by ZnO catalyst," Dyes and Pigments, vol.
Soon after sputum sample collection, smear microscopy was done by using florescence microscopy (FM) and Auramine
O staining procedure which is described as follows: first a sputum smear was prepared and allowed to air-dry; then this smear was heat-fixed.
Microscopy of gastric washings for acid-fast bacilli using the auramine
stain was negative.