To test the assay specificity, we used a number of viruses from our strain collection, including avian nephritis virus (Astroviridae
); QX-like, 4/91-like, and Massachusetts-like infectious bronchitis virus strains (Coronaviridae); representative isolates of avian metapneumovirus A, B, and C and Newcastle disease virus (Paramyxoviridae); isolates of influenza (H9N2) and (H5N8) viruses (Orthomyxoviridae); and Tembusu virus (Flaviviridae).
Los astrovirus (AstV), pequenos virus con genoma de ARN de cadena simple, de la familia Astroviridae
, son la causa mas comun de gastroenteritis no solamente en ninos pequenos (menores de 2 anos) inmunocompetentes, sino tambien entre los ancianos y pacientes inmunodeficientes (44).
(13,14) The Astrovirus (AstV), Astroviridae
family, a small round virus, has been associated with AGE, with a frequency range between 2% to 26%.
Los VE comunmente asociados con enfermedad en humanos pertenecen a las familias Adenoviridae (Adenovirus), Caliciviridae (Norovirus, Sapovirus), Picornaviridae (Enterovirus, virus hepatitis A y Echovirus), Reoviridae (Reovirus y Rotavirus), y Astroviridae
The family Astroviridae is currently divided into 2 genera: the genus Mamastrovirus of mammal-infecting viruses and the genus Avastrovirus of avian viruses (3,4).
Astroviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ, editors.
Astroviruses, family Astroviridae
, are small, nonenveloped, single-stranded RNA viruses.
The family Astroviridae
comprises 2 genera, Mamastrovirus and Avastrovirus, known to infect mammals and birds, respectively.
The implications of these findings were obscured by the fact that the brain homogenate also included sequences with partial analogy to viruses of the family Astroviridae
and family Retroviridae.
Astroviruses (AstVs) (family Astroviridae
) are nonenveloped, and their genome is composed of a plus-sense single-stranded RNA of 6.4-7.3 kb, containing 3 open reading frames (ORFs) and a 3' poly-A tail (1).
Five microliters of RNA/DNA eluate were tested by broad range reverse transcription-PCR (RT-PCR) assays for the whole subfamily Coronavirinae (16), the family Astroviridae
(4), and the genus Mastadenovirus (18).
Human astroviruses (HAstVs) belong to the Astroviridae
family, which is divided into 2 genera: Mamastrovirus (mammalian viruses) and Avastrovirus (avian viruses) (1).