asparagine ligase

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as·par·a·gine li·gase

an acid:ammonia ligase (amide synthetase) forming l-asparagine and l-glutamate from l-aspartate and l-glutamine, with the concomitant cleavage of ATP to AMP and pyrophosphate. Under nonphysiological conditions, the mammalian enzyme can use ammonia as the nitrogen donor. Asparagine ligase also displays a glutaminase-like activity.
References in periodicals archive ?
In normal cells, oxaloacetate is converted to aspartate by transamination which further converts into L-asparagine by asparagine synthetase which converts the carboxy group to amide group in the side chain.
Asparagine can be synthesized from aspartate and glutamine (or ammonia) by asparagine synthetase in mammals [5,6].
, the team discovered that the appearance of asparagine synthetase -- the enzyme cells used to make asparagine -- in a primary tumour was strongly associated with later cancer spread.
Asparagine synthetase (ASNS) is an expression status present in various tumour types, such as leukaemia, lymphoma and pancreatic cancer.
Of these, expansin, b-xyloglucan endotransglycosylase (XET), acyl-CoA-binding protein, asparagine synthetase, and seed storage protein were up- regulated during the early development period and reduced sharply at the late development stage.
The team identified the gene affected by the mutation code for asparagine synthetase, the enzyme responsible for synthesising the amino acid asparagine.
In order to assess the applicability of the recommended histone H1, [alpha]TUB, and GAPDH as reference genes in relative expression studies using RT-qPCR, four targets based on sugarcane SuperSAGE unitags annotated as glutamine-dependent asparagine synthetase (AS, EC 6.3.5.4), pyrophosphate fructose-6phosphate 1-phosphotransferase alpha subunit (PFP[alpha]1, EC 2.7.1.90), plasma membrane intrinsic protein (PIP1-1), and 1aminocyclopropane-1-carboxylate oxidase (ACC oxidase, EC 1.14.17.4) were evaluated (Table 4).
Washington, Dec 2 ( ANI ): The deficiency of asparagine synthetase, which is found in meat, eggs and dairy products, caused by rare genetic disorder affects development of the brain.
Coruzzi, "Metabolic regulation of the gene encoding glutamine-dependent asparagine synthetase in Arabidopsis thaliana," Plant Physiology, vol.
Asparagine is considered a nonessential amino acid because, while noncancerous cells contain asparagine synthetase, malignant lymphocytes often lack this ability and are dependent on extracellular asparagine.
The DNA sequence and characteristics of cDNA clones encoding cytosolic (Gebhardt et al., 1999) and plastidic aspartate aminotransferase (Wadsworth et al., 1993), aspartokinase homoserine dehydrogenase (Gebhardt et al., 1998), dihydrodipicolinic acid synthase (Silk et al., 1994), asparagine synthetase (Hughes et al., 1999), and a 34-kDa protein (Kalinski et al., 1990) were reported previously.
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