LPL is also involved in promoting the cellular uptake of free fatty acids, cholesterol-rich lipoproteins, and chylomicron remnants while ApoC-II
acts as a cofactor of LPL (Tian et al., 2012).
Apolipoprotein C-III is a potent inhibitor, whereas the apolipoprotein C-II is an activator of lipoprotein lipase, and for the normal functioning lipoprotein lipase requires the presence of apoC-II
, as a cofactor .
ativa a LPL para que o TAG sofra hidrolise e libere acidos graxos nos tecidos adiposo e muscular.
Gel bands containing apoB100, apoE, and comigrating apoC-I, apoC-II, and apoC-III were excised with an EXQuest Spot Cutter (Bio-Rad).
This approach was repeated to establish cutoff values for the peptides studied (peptides from apoB100, apoE, apoC-II, apoC-III, CETP, and PCSK9), which are listed in online Supplemental Table 1.
To apply this method to other proteins involved in lipoprotein metabolism, we measured [D.sub.3]-leucine incorporation into the apoE, apoC-II, apoC-III, CETP, and PCSK9 peptides (see Supplemental Table 1) by LCSRM using the cutoffvalues described earlier.
Chylomicronaemia may also be due to apoC-II
deficiency that is also recessively inherited but may be clinically less severe than LPL deficiency and could respond to fresh plasma infusion.
For instance, some cases of familial chylomicronemia result from mutations in the gene encoding lipoprotein lipase or its cofactor apoC-II. Some cases of familial combined hyperlipoproteinemia result from mutations in the upstream transcription factor 1 (USFI) gene encoding a transcription factor.
* In addition to heterozygous E3[DELTA]149Leu, the differential diagnosis of nontraumatic splenomegaly with hypertriglyceridemia includes: deficiency of lipoprotein lipase or its cofactor apoC-II, deficiency of lysosomal acid lipase, or hemophagocytic lymphohistiocytosis (1-3).
Quantification of apoC-II
and apoC-III of human very low density lipoproteins by analytical isoelectric focusing.
Additionally, we found that lipid metabolism-associated DEPs, including ApoC-II
, Apo(a), cholesteryl ester transfer protein(CETP), and LPL, were significantly altered in the TBI patients with cognitive impairment.