1: more than an anti-phospholipase protein," Inflammation Research, vol.
V, propidium iodide and Hoechst staining: For Annexin
V, staining suspensions of cumulus cells from individual oocytes were incubated with 1 [micro]l of Annexin
V (2.5 [micro]g/ml) for 15 min at 37 [degrees]C in the dark.
Early apoptotic cells can easily be identified by green fluorescence of FITC-conjugated annexin
V, as annexin
V has a high affinity toward phosphatidyl serine (PS) residues which are externalized from inner to outer leaflet of the plasma membrane during early stages of apoptosis [57, 58].
Indeed, aPL represent a heterogeneous family of antibodies reacting with phospholipid-binding cofactor proteins, including not only [[beta].sub.2]-GPI [9, 10] but also different anionic phospholipids, proteins, or phospholipid-protein complexes, such as prothrombin , protein S [12, 13], protein C , annexin
V , annexin
II , oxidized low-density lipoprotein, lysobisphosphatidic acid (LBPA), and sulfatides [17-19].
We recently demonstrated overproduction of annexin
A1 (ANXA1) in the urine in a mouse Adriamycin-induced glomerulopathy (ADG) model, featuring both chronic glomerulosclerosis and tubulointerstitial damage, by using the two-dimensional electrophoresis gel and matrix-assisted laser desorption ionization/time of flight mass spectrometry analysis .
Cells were harvested and resuspended with PBS containing FITC-labeled Annexin
V/propidium iodide mixture.
Early apoptotic cells can easily be identified by green fluorescence of FITCconjugated annexin
V as annexin
V has a high affinity towards phosphatidylserine (PS) residues which are externalized from inner to outer leaflet of the plasma membrane during early stages of apoptosis [51, 52].
Athera Biotechnologies AB, has transferred the rights to the recombinant Annexin
A5 to Medirista AB.
This study investigates the utility of 26 different immunohistochemical markers (CAM 5.2, CK [cytokeratin] 7, CK20, CK17, CK19, MUC1, MUC2, MUC4, MUC5AC, MUC6, p53, DPC4/ SMAD4, CDX2, pVHL [von Hippel-Lindau tumor suppressor gene protein], S100P, IMP-3 [insulin-like growth factor 2 messenger RNA binding protein 3], maspin, mesothelin, claudin 4, claudin 18, annexin
A8, fascin, PSCA [prostate stem cell antigen], MOC31, CEA [carcinoembryonic antigen], and CA19-9 [cancer antigen 19-9]) in the diagnosis of ductal adenocarcinoma of the pancreas by using 60 cases of pancreatic DAC on routine and tissue microarray (TMA) sections.
Inhibition of late apoptosis by estrogen: Flow cytometry data in Figure 4AFigure 4D shows that 100[micro]M [H.sub.2][O.sub.2] increased Annexin
V and propidium iodide positive cells on a time dependent manner.
Cells were stained with annexin
V and PI to determine the nimbolide-induced apoptotic death of HT-29 cells.
All fluorescence observations were made with a Zeiss Standard 14 photomicroscope with epifluorescence system equipped with an automatic C 35 camera loaded either with Kodak Gold 100 ASA film, for slides stained with acridine orange (AO) and ethidium bromide (EB), or with Kodak Ultra 400 ASA, for slides strained with JC-1 and Annexin