Ang-2 expression can be regulated by hypoxia inducible factors and other regulators with exposure to hypoxia.
Ang-1 promotes vascular stabilization and counteracts VEGF-induced angiogenesis whereas Ang-2 promotes angiogenesis by reducing the destabilization of endothelial cell junctions to enhance new vessel branching and sprouting.
sup] Ang-2 plays a crucial role in this hypoxia-induced angiogenesis, and increased levels of Ang-2 concentrations can be detected in the peripheral blood of patients with CHD.
Given this information, we hypothesized that serum Ang-2 concentrations in patients with CHD might decrease after myocardial blood reperfusion by percutaneous coronary intervention (PCI) and the greater decreases in Ang-2 after PCI accompany more severe coronary artery stenosis.
Ang-2 concentrations in the blood samples were measured using enzyme-linked immunosorbent assay kits according to the manufacturer's instructions (RayBiotech, Inc.
Pre-PCI serum Ang-2 concentrations in the study group were significantly higher than those in the control group (4625.
Materials and Methods: Ang-2 and Tie-2 were measured in plasma samples from AML patients and controls using enzyme-linked immunosorbent assay (ELISA).
Yontem ve Gerecler: AMLli hastalar ve kontorllerden alinan plazma orneklerinde, Enzim bagli immunassay (ELISA) ile Ang-2 ve Tie -2 olculmustur.
sonuc: Calismada elde edilen sonuclarda, AML hastalarinin dolasimdaki Ang-2 ve sTie-2 duzeylerinin prognostik anlamliligi gosterilmistir.
Ang-1 and its naturally occurring antagonist Ang-2 act via the Tie-2 receptor tyrosine kinase, which is broadly expressed in the endothelium of the adult vasculature and in a subset of hematopoietic stem cells.
Plasma levels of Ang-2 and Tie-2 were assayed by enzyme-linked immunosorbent assay (ELISA) using commercially available kits (Quantikine, R&D systems) according to the manufacturer*s instructions.
Medians, means and ranges of plasma levels of Ang-2 and Tie-2 in AML patients and healthy controls are presented in Table 2.