Plasma angiopoietin-2 (ANG-2) is a proinflammatory cytokine that can regulate endothelial permeability .
Plasma concentrations of ANG-2, sE-selectin, IL-6, and IL-8 were measured by commercial ELISA kits (Cusabio, China) according to the manufacturer's instructions as follows: standards for ANG-2, sE-selectin, IL-6, and IL-8 were prepared for generating corresponding standard curves.
For the establishment of the model with the LIPS and ANG-2, the probability value (P value) was obtained from logistic regression analysis and then used as a new indicator for the diagnosis of ARDS based on receiver operating characteristic (ROC) curve analysis.
Prediction and Regression Analysis of LIPS, APACHE2 Score, and ANG-2, sE-Selectin, IL-6, and IL-8 Levels for ARDS
LIPS, APACHE2 Score, and ANG-2, sE-Selectin, IL-6, and IL-8 Concentration in the ARDS and Non-ARDS Groups.
Univariate analysis showed that ANG-2 level, sE-selectin level, APACHE2 score, LIPS, and septic shock were closely associated with ARDS (Table 4, univariate analysis).
Background: Angiopoietin-2 (Ang-2) plays a crucial role in hypoxia-induced angiogenesis and is expressed only in sites of vascular remodeling.
Several growth factors are closely associated with blood vessel formation and remodeling, such as angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), and vascular endothelial growth factor (VEGF), [sup] each of which plays a different role in angiogenesis.
[sup] Ang-2 plays a crucial role in this hypoxia-induced angiogenesis, and increased levels of Ang-2 concentrations can be detected in the peripheral blood of patients with CHD.
Given this information, we hypothesized that serum Ang-2 concentrations in patients with CHD might decrease after myocardial blood reperfusion by percutaneous coronary intervention (PCI) and the greater decreases in Ang-2 after PCI accompany more severe coronary artery stenosis.
Ang-2 concentrations in the blood samples were measured using enzyme-linked immunosorbent assay kits according to the manufacturer's instructions (RayBiotech, Inc., Norcross, GA, USA).
Pre-PCI serum Ang-2 concentrations in the study group were significantly higher than those in the control group (4625.06 [+ or -] 1838.06 vs.