DNA replication

(redirected from Amplification of DNA)

DNA replication

The formation of new and, hopefully, identical copies of complete genomes. DNA replication occurs every time a cell divides to form two daughter cells. Under the influence of enzymes, DNA unwinds and the two strands separate over short lengths to form numerous replication forks, each of which is called a replicon. The separated strands are temporarily sealed with protein to prevent re-attachment. A short RNA sequence called a primer is formed for each strand at the fork. These primers provide a free 3'-OH end on which the new complementary sequence can be formed along the strand. The LEADING STRAND is synthesized continuously in the 5’ to 3’ direction, working towards the fork direction with removal of the RNA primers as the parental duplex is unwound. The LAGGING STRAND is synthesized discontinuously in the opposite direction as short fragments called Okazaki fragments. Lagging strand synthesis requires extension of the primer, then removal of the primers and gap filling. At least 20 different enzymes and factors, including DNA helicases, DNA polymerases, RNA primases, DNA TOPOISOMERASES and DNA ligases are involved in the complex process of DNA replication.

DNA replication

see DNA.
References in periodicals archive ?
Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000; 28: E63, doi: 10.1093/nar/28.
Loop-mediated isothermal amplification of DNA is a method that has gained momentum in the diagnosis of different microorganisms due to its inherent advantages of high sensitivity and specificity and its potential applicability in resource-poor endemic areas [20].
The ADSRRS method (Amplification of DNA Surrounding Rare Restriction Sites) is a modern molecular analytic method developed by Phicienniczak and Masny, based on supression of amplification of parts of fragments in PCR reaction [11].
Visual detection of potato leafroll virus by one-step reverse transcription loop-mediated isothermal amplification of DNA with hydroxynaphthol blue dye.
Polymerase Chain Reaction (PCR) is a technique that allows for selective amplification of DNA sequences.
The BD ProbeTec T vaginalis Qx Amplified DNA Assay (Becton Dickinson; Franklin Lakes, NJ) is also approved for detection of T vaginalis, and it relies on amplification of DNA rather than RNA.
Alternative amplification of DNA by loop-mediated isothermal amplification, using the 529 element, has been determined [10,25] and should be developed further for application in point-of-care diagnosis.
Mystaire's MY-PCR Prep workstations provide laboratory technicians with a "personal cleanroom" for use in the amplification of DNA and RNA.
Touchdown PCR--DNA quality and quantity obtained from soil samples was variable; therefore, we employed touchdown PCR as a sensitive and highly specific method for the amplification of DNA from Geomyces and close relatives (Don et al., 1991; Lindner and Banik, 2009).