Amino Acid Sequence
: The N-terminal amino acid sequence
of purified globin chains was deduced by Edman degradation in an automated sequencer; model Procise 491 (Applied Biosystems).
Based on such research, Fujitsu Laboratories, the Insight Centre, and Fujitsu Ireland have now included not only structural information about amino acid sequences
in the knowledge graph, but also chained information.
Mass Spectrometry and Amino Acid Sequence
. Pentocin JL-1 was identified by MALDI-TOF-MS.
Sequencing of the PCR product and prediction of amino acid sequence
2004) and Expasy Bioinformatics Resource Portal (http://web.expasy.org.translate) for phylogenetic and amino acid sequence
In this study, evolutionary trace (ET) method which relies on both amino acid sequence
and structural information to analyze functional sites of proteins is used because of the advantage of having molecular and structural information in a single analysis 11].
The phylogenetic tree map was drawn by DNAMAN software on the basis of amino acid sequence
of GAPDH in 6 species.
An example of amino acid sequence
similarity resulting from sequence comparisons across biology is represented in Figure 1.
Scientists are creating these variants by modifying the amino acid sequence
For example, 97 percent of the amino acid sequence
in human collagen matches that of the cow.
Table 1: ICH Topic Q6 B (Specifications: test procedures and acceptance criteria for biotechnological/biological products) Appendix for Physiochemical Characterization Structural characterization and confirmation a) Amino acid sequence
b) Amino acid composition c) Terminal amino acid sequence
d) Peptide map e) Sulfhydryl groups and disulphide bridges f) Carbohydrate structure Physicochemical properties --Molecular weight or size --Isoform pattern --Extinction coefficient --Electrophoretic patterns --Liquid chromatographic patterns --Spectroscopic profiles
When operated in ETD mode, the instrument provides extensive and easy-to-interpret amino acid sequence
information in the form of complementary c- and z-series ions.