* The report reviews Lysine-Specific Histone Demethylase 1A (Lysine (K)-Specific Demethylase 1 or Flavin-Containing Amine Oxidase
Domain-Containing Protein 2 or EC 1.) targeted therapeutics under development by companies and universities/research institutes based on information derived from company and industry-specific sources
Shevchenko, Thermostable AY- cyclodextrin conjugates of two similar plant amine oxidases
and their properties, Biotechnol.
Welford et al., "Exploring molecular oxygen pathways in Hansenula polymorpha copper-containing amine oxidase
," Journal of Biological Chemistry, vol.
The enzymes used in their work include amino acid-lyases, (48)-(57) aromatic amino acid decarboxylases, (58)-(64) and amine oxidases
. (65)-(72) When they started their work, enzymes were not routinely purified or particularly well characterized.
The amine oxidase
preparation was stable at -18[degrees]C and there was no detectable loss of activity in 6 months at this temperature.
Studying a bovine amine oxidase
, two independent research groups found clues that pyrroloquinoline quinone (PQQ) might be the enzyme's missing sidekick in cows -- and perhaps in humans as well, Klinman says.
Aminoguanidine and pentamidine (amine oxidase
inhibitors), as well as NAC (an antioxidant) and ALDH (an aldehyde-quenching enzyme), suppressed spermidine-induced ARPE-19 cell death in our in vitro study, and the latter two agents also ameliorated RPE dysfunction and death in our in vivo experiments.
The copper-containing amine oxidases
(monoamine oxidase, semicarbazide-sensitive amine oxidase
, histamine deaminase, amine oxygen oxidoreductase) are ubiquitous metalloenzymes as they have several essential functions in the metabolism of primary amines.
Acrolein is the principal cytotoxic metabolite of the anticancer and arthritis drug cyclophosphamide and is the principal and most physiologically active metabolite of allylamine (15), which is metabolized intracellularly to acrolein via semicarbazide sensitive amine oxidase
. The activity of this enzyme is high in artery wall cells (16).
In developing the biosensor, they used the enzymes diamine oxidase, monoamine oxidase and plasma amine oxidase
as the specific biological recognition compounds in a continuous flow system.
The AO family encompasses FAD-dependent enzymes (essentially MAO and polyamine oxidase) and copper-containing amine oxidases
, having for predominant members the products of the genes AOC1, 2, and 3, the latter being named semicarbazide-sensitive amine oxidase
(SSAO) or primary amine oxidase
and also currently known as vascular adhesion protein-1 (SSAO/VAP-1) [5,6].