After electrophoretic blotting on a nitrocellulose membrane (BA 85; Schleicher & Schuell, Dassel, Germany), the membrane was subjected to amido black staining and immunological detection to assess the effect of Es deficiency on CA.
Amido black staining resulted in appearance of smear protein bands corresponding to molecular weights of 31-43 kD and disappearance of the bands corresponding to some high-molecular-weight proteins (Fig.
Precipitated protein spots were stained either with amido black
alone or in combination with other protein stains.
ID and IEP were performed in 0.9% agar gel and the results were recorded in fresh preparations and after staining with amido black. IMB was performed, using both native (without detergents) and SDS-PAGE gels as previously described (5).
After separation, one gel was fixed for an hour using a fixative solution (40 vol %Ethanol; 2 vol %acetic acid) and stained with amido black in order to visualise the [alpha]2M band.
Unprecipitated proteins were washed away before staining of the precipitated complexes with Amido black
Diaminobenzidine (DAB); if not available, use amido black
Diaminobenzidine (DAB); if not available, use leucocrystal violet (LCV) or amido black
is used to develop latent prints and enhance visible prints that have been deposited in blood.
We studied a 62-year-old man with light chain disease (multiple myeloma) whose urine protein electrophoresis (UPE; electrophoresis performed on agarose gel on a Panagel high resolution electrophoresis system; gel was stained with Amido Black
) showed >15 narrow bands in the [beta]- and [gamma]-globulin regions; many of the bands were strongly stained and equidistant (Fig.
Cyanoacrylate fuming may be detrimental to the amido black
This amido black
water-based formula is a one-step process that includes a blood fixer.