Ames test


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Ames test

 [āmz]
a test for mutagenic substances, in which a strain of Salmonella typhimurium that lacks the enzyme necessary for histidine synthesis is cultured in the absence of histidine and in the presence of the suspected mutagen. If the substance causes DNA damage resulting in mutations, some of the bacteria will regain the ability to synthesize histidine and will proliferate to form colonies.

Ames test

(āmz),
a screening test for possible carcinogens using strains of Salmonella typhimurium that are unable to synthesize histidine; if the test substance produces mutations that regain the ability to synthesize histidine, the substance is carcinogenic.
Synonym(s): Ames assay

Ames test

n.
A test used to determine the mutagenic potential of a substance, in which salmonella bacteria that are unable to synthesize histidine are introduced into the substance, and the substance is deemed mutagenic and carcinogenic if the bacteria regain the ability to synthesize histidine.

Ames test

A bioassay that detects genetic mutations, which is used to screen for compounds with carcinogenic potential.

Ames test

(āmz test)
A screening procedure for possible carcinogens using strains of Salmonella typhimurium that are unable to synthesize histidine; if the test substance produces mutations that regain the ability to synthesize histidine, the substance is carcinogenic.

Ames test

or

mutatest

a technique devised in the USA by Bruce Ames and an associate that is designed to screen environmental chemicals for mutagenicity. The test generally uses histidine-requiring mutant strains of the bacterium Salmonella typhimurium as the ‘tester strains’ and measures the frequency of BACK MUTATIONS where the mutants no longer require histidine supplements in their food supply. Many of the tester strains also have mutations that increase permeability to certain chemicals and enhance mutagenic effects by being defective in DNA REPAIR systems. The Ames test has been employed widely since 1975 as a check for potential CARCINOGENS, since these chemicals usually act as MUTAGENS.

Ames,

Bruce N., U.S. molecular geneticist, 1928–.
Ames assay - a screening test for possible carcinogens. Synonym(s): Ames test
Ames test - Synonym(s): Ames assay
References in periodicals archive ?
All genotoxicity data collected from the literature consultation and the Ames test were finally combined to assign each of the 106 substances to one of the following priority classes:
typhimurium Ames test, which renders it suitable for further studies because it possesses therapeutic uses.
Comparison of the Ames II and traditional Ames test responses with respect to mutagenicity, strain specificities, need for metabolism and correlation with rodent carcinogenicity.
Rat fed with milk cultured with Enterococcus faecium IS-27526 significantly decreased fecal as well as urinary mutagenicity of rats as measured by the Ames test (Tables 3 and 4).
The original Ames test was in the public domain, but UC Berkeley has a patent pending for Ames II.
Since the databases of the models used in this study were built using experimental data from the Ames test, we only considered data for mutagenicity obtained with this assay, even if other in vitro or in vivo tests gave different results for the substances under examination.
Tests were started with the first step of the test strategy, the Ames test, in five bacterial strains.
BACKGROUND: Many bacterial or mammalian cell-based test systems, such as the Ames test, chromosomal aberration assays, or gene mutation assays, are commonly used in developed countries to detect the genotoxicity of industrial chemicals.
What's more, Petrakis says, secretion from "8 percent or so of the women came up positive in the Ames test," a bacterial assay to identify possible carcinogens.
A prime example is the Ames test, which was developed on the foundation of understanding the basic science of mechanisms of mutagenesis and of the role of mutations in cancer.
Antimutagenic activity of green tea (Camellia sinensis) was studied using Salmonella typhimurium strains (TA 102) (Ames test).