Ames test

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Ames test

 [āmz]
a test for mutagenic substances, in which a strain of Salmonella typhimurium that lacks the enzyme necessary for histidine synthesis is cultured in the absence of histidine and in the presence of the suspected mutagen. If the substance causes DNA damage resulting in mutations, some of the bacteria will regain the ability to synthesize histidine and will proliferate to form colonies.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.

Ames test

(āmz),
a screening test for possible carcinogens using strains of Salmonella typhimurium that are unable to synthesize histidine; if the test substance produces mutations that regain the ability to synthesize histidine, the substance is carcinogenic.
Synonym(s): Ames assay
Farlex Partner Medical Dictionary © Farlex 2012

Ames test

n.
A test used to determine the mutagenic potential of a substance, in which salmonella bacteria that are unable to synthesize histidine are introduced into the substance, and the substance is deemed mutagenic and carcinogenic if the bacteria regain the ability to synthesize histidine.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

Ames test

A bioassay that detects genetic mutations, which is used to screen for compounds with carcinogenic potential.
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

Ames test

(āmz test)
A screening procedure for possible carcinogens using strains of Salmonella typhimurium that are unable to synthesize histidine; if the test substance produces mutations that regain the ability to synthesize histidine, the substance is carcinogenic.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

Ames test

or

mutatest

a technique devised in the USA by Bruce Ames and an associate that is designed to screen environmental chemicals for mutagenicity. The test generally uses histidine-requiring mutant strains of the bacterium Salmonella typhimurium as the ‘tester strains’ and measures the frequency of BACK MUTATIONS where the mutants no longer require histidine supplements in their food supply. Many of the tester strains also have mutations that increase permeability to certain chemicals and enhance mutagenic effects by being defective in DNA REPAIR systems. The Ames test has been employed widely since 1975 as a check for potential CARCINOGENS, since these chemicals usually act as MUTAGENS.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

Ames,

Bruce N., U.S. molecular geneticist, 1928–.
Ames assay - a screening test for possible carcinogens. Synonym(s): Ames test
Ames test - Synonym(s): Ames assay
Medical Eponyms © Farlex 2012
References in periodicals archive ?
The Ames assay is well established and an excellent method for screening of such cancer chemopreventive factors (Brockman et al.
Citrus fruits, for example, contain certain chemicals that appear carcinogenic in the Ames assay, but an assessment of epidemiologic studies led a National Research Council committee to conclude last March that these fruits may actually help protect people from stomach cancer.
1531) estimated associations between the concentrations of four trihalomethanes (THMs) in exhaled breath of swimmers and changes in biomarkers before and after swimming, including micronuclei (MN) and DNA damage (comet assay) in peripheral blood lymphocytes; urine mutagenicity (Ames assay); and MNs in exfoliated urothelial cells.
Dimethylnitrosamine (DMN) -- a known animal carcinogen -- is usually a potent mutagen in the Ames assay. but when the Eppley researchers conducted an Ames assay on paired exposure to both DMN and capsaicin, "we found the mutagenicity of the DMN was effectively destroyed," Gannett says.