The chondrogenic differentiation potential was examined using a pellet culture, with the pellet size monitored and the degree of
alcian blue staining observed.
Tecnica G1 PAS 791,8 [+ or -] 177,7 ([section])# AB pH 2,5 645,2 [+ or -] 114,1 AB pH 1,0 695,5 (607,8; 761,0) Tecnica G2 PAS 1116,0 [+ or -] 175,9([section]) AB pH 2,5 731,2 [+ or -] 223,8 AB pH 1,0 709,2 (604,9; 734,1) Tecnica G3 PAS 1084,2 [+ or -] 85,1# AB pH 2,5 800,5 [+ or -] 138,9 AB pH 1,0 732,2 (660,1; 836,8) PAS: Periodic-Acid-Schiff; A.B:
Alcian Blue. Valores expressos como media (+ or -] desvio padrao ou mediana (P25; P75).
The stomach contents were immersed immediately in 10 ml of 0.02%
Alcian blue in 0.16 M sucrose/0.05 M sodium acetate, pH 5.8, and incubated for 24 h at 20 [degrees]C.
Next, we performed
Alcian blue and Sirius red staining for cartilage proteoglycan collagen, respectively.
Depletion of intestinal goblet cells was observed in 10 cases of PCV2 IHC-stained sections of colon counterstained with
Alcian blue and hematoxylin.
The results of alizarin red, oil red O, and
alcian blue stainings showed that osteogenic, adipogenic, and chondrogenic differentiations of BMSCs were all enhanced in the DRG+ BMSC group, compared with the BMSC group (Figure 2(c)).
The edematous stem villous stroma stains for
Alcian blue, but is negative for smooth muscle actin, which shows positive staining of normal villous stroma.
On special stain (
alcian blue) there was some mucin deposition in areas of degenerated collagen.
Silver staining combined with
alcian blue and hematoxylin-eosin for the detection of Lawsonia intracellularis in swine proliferative enteropathy.
After routine histological methods, 5 [micro]m sections were obtained and coloured by Gomori's trichrome, periodic acid-Schiff (PAS),
Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide + PAS.
The sections were first cleared of paraffin using xylene and rehydrated in gradients of alcohol and then stained with
Alcian blue (Sigma) in 3% acetic acid; the sections were then stained with
Alcian blue and then dehydrated with 95% ethyl alcohol, 100% ethyl alcohol, and xylene.
The staining currently automated are: Massons~s trichrome (or Gomori~s trichrome), Grocott~s Methenamine Silver (or Gomori~s Methenamine Silver), Giemsa, Gordon and Sweet (or Gomori and Snook), Congo Red, Jones Methenamine Silver (with H&E contrast colour), Gram, PAS + diastase (2 kit), Perls Prussian Blue,
Alcian Blue (pH 2,5), Ziehl-Neelsen, Elastin (Modified Weigert~s Resorcin Fuchsin, Verhoeff~s elastic tissue).
