Alcian Blue Stain


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A proprietary water-soluble phthalocyanin dye CI 74240 used in histology, which can be modified at different pHs to identify specific mucopolysaccharide families
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D) Cryptococcal organisms and the surrounding polysaccharide capsules stained blue with Alcian Blue stain.
Histopathological examination using the Alcian blue stain revealed mucin deposition in the reticular dermis with connective tissue separation [Figure 1]d.
PAS and Alcian blue stain were useful to differentiate adenocarcinoma, mucinous adenocarcinoma and signet ring cell carcinoma.
Other stains can be applied according to the need of diagnosis, for example, Gram's stain, Ziehl-Neelsen stain, Periodic acid-Schiff stain, Alcian blue stain, and Papanicolaou stain.
Histological cutaneous examination revealed epidermal atrophy, and superficial and deep perivascular and perifollicular dermatitis with focally increased dermal mucin highlighted by Alcian blue stain, suggesting lupus erythematosus (LE).
At incubation intervals of 24, 48 and 72 hours, all experimental groups were stained with hematoxylin and eosin stain (H-E) and alcian blue stain.
Chondrogenic differentiation was confirmed with Alcian blue stain kit (NovaUltra[TM], IHC World, USA) by detecting glycosaminoglycan stained in blue color.
Alcian Blue stain was used for 10um-thick sections to demonstrate proteoglycan content.
The recognition of intestinal metaplasia, especially in goblet cells, is increased by the use of Alcian blue stain.
Highlighting the hyaluronic acid-rich matrix of medulloepithelioma with Alcian blue stain may aid the diagnosis of medulloepithelioma, particularly in cases that are cellular or heavily pigmented (Figure 3, inset).