After 14 days of cultivation, chondrogenic pellets were stained with Alcian Blue Stain
Inset, fungal capsule exhibits marked Alcian Blue stain
Slides were stained with hematoxylin and eosin and alcian blue stain
. Osteocytes were counted in a defined unit area under high power in the two groups and the average of the two groups was compared.
Caption: FIGURE 3: Alcian blue stain
showing increased dermal mucin deposition (Alcian blue; original magnification x10).
Rehydrated tissue sections were immersed in Alcian blue stain
solution (Alcian blue 8GX, 1 gm / 100 mL 3% acetic acid pH 2.5, Sigma-Aldrich, St.
It stains with alcian blue stain
at pH 2.5 and is susceptible to hyaluronidase digestion.
Histopathological examination using the Alcian blue stain
revealed mucin deposition in the reticular dermis with connective tissue separation [Figure 1]d.
(b) Intracellular and extracellular mucin deposition (focal distribution) is present (Alcian blue stain
, original magnification x400).
After staining, extracellular matrix was taken to dark blue stain where the so-called Alcian blue stain
had metachromatic appearance to extracellular matrix (Figure 5(a)).
(a) A palisading granuloma with central collagen degeneration (H&E stain, magnification x100); (b) Alcian blue stain
showing increased mucin deposition within the granuloma (Alcian blue stain
, magnification x100); (c) several annular red-brown plaques involving the thigh.
Other stains can be applied according to the need of diagnosis, for example, Gram's stain, Ziehl-Neelsen stain, Periodic acid-Schiff stain, Alcian blue stain
, and Papanicolaou stain.
Histological cutaneous examination revealed epidermal atrophy, and superficial and deep perivascular and perifollicular dermatitis with focally increased dermal mucin highlighted by Alcian blue stain
, suggesting lupus erythematosus (LE).