AlamarBlue assay

AlamarBlue assay

An assay used to quantify the proliferation of various human and animal cell lines, bacteria and fungi, and assess relative cytotoxicity of agents within various chemical classes. The assay is based on detection of metabolic activity through an oxidation-reduction (REDOX) indicator, which both fluoresces and changes colour in response to chemical reduction of growth medium resulting from cell growth.

It is simple to perform because the indicator is water soluble, thus eliminating the washing/fixing and extraction steps required in other commonly used cell proliferation assays.
References in periodicals archive ?
The collagen scaffolds were seeded with fibroblasts; AlamarBlue assay showed approximately 60% cell attachment after seeding and cell proliferation in the next two weeks.
The evaluation of growth and viability of cells was done by AlamarBlue Assay. The insert with sample was discarded, and AlamarBlue reagent was added to the medium with cells and incubated for 4 h at 37[degrees] C.
Caption: Figure 13: Number of viable rMSCs after 72 h of growth on BC-PHEMA composites determined by cell viability AlamarBlue Assay.
The effect of TGF[beta]1 on hMSC cell viability was assessed using alamarBlue assay reagent.
Cell viability was measured using alamarBlue assay reagent (AbD Serotec, Raleigh, NC, USA) according to manufacturer's recommendations.
AlamarBlue assay measures the cell capability to convert resazurin into resorufin, providing insight of cell health and viability.
The alamarBlue assay was performed at the third and sixth days (3 or 6 days) of treatment in both HIV-1-infected and uninfected THP1 cells.
The cell viability was determined using the AlamarBlue assay (Invitrogen, Germany).
The AlamarBlue assay was used to examine the cellular metabolic activity when the cells were exposed to the low (200 [micro]l of [H.sub.2]O with 50 [micro]l of DMEM) and high (10 [micro]l 31% NaCl solution with 100 [micro]l of DMEM) salinity challenges.
To quantitatively evaluate the E1 cells adhered to the pearl disks over time, an alamarBlue assay kit (AbD Serotec, Oxford, UK) was used.