The present study was taken up to study the aged related variations in total leucocyte, granulocyte and agranulocyte count during different phases of menstrual cycle in 1st professional MBBS students at Jawaharlal Nehru Institute of Medical sciences (JNIMS), Imphal, Manipur.
However, rise in granulocyte, agranulocyte and total leucocyte counts may be due to the release of tremendous number of leucocytes during menstruation.
Comparison of Granulocyte & Agranulocyte Count in Different Phases of Menstrual Cycle in Different Age Groups Granulocyte No.
DEGs in agranulocyte adhesion and diapedesis, granulocyte adhesion and diapedesis, IL-10 signaling, and IL-6 signaling were further analyzed and illustrated by the Venn diagram.
Further IPA canonical pathway analysis reveals that significantly dysregulated pathways in immune/inflammatory reactions following injury were agranulocyte adhesion and diapedesis, granulocyte adhesion and diapedesis, IL-10 signaling, and IL-6 signaling (Table 1, Additional Files 5, 6, 7, 8, 9, 10, 11, and 12) based on the score (-log (p value)) of each pathway (p value < 0 05 were considered statistically significant).
When analyzing the DEGs at different time points, we found 46, 46, 26, and 24 genes commonly upregulated or downregulated in the pathways of agranulocyte adhesion and diapedesis, granulocyte adhesion and diapedesis, IL-10 signaling, and IL-6 signaling, respectively (see the intersect in red, Figures 2(a), 2(b), 2(c), and 2(d)).
Signaling pathway analysis suggested that agranulocyte adhesion and diapedesis, granulocyte adhesion and diapedesis pathway, IL-6 signaling, and IL-10 signaling are the major immune/inflammation pathways upregulated during nerve degeneration and regeneration.
The most important reviews of the various morphofunctional aspects of the hemocytes of the whole Mollusca phylum are those of Cheng (1981) and Hine (1999), who identified 2 fundamental hemocyte types in bivalve hemolymph: granulocytes and hyalinocytes (or agranulocytes).
On Hemacolor smears, 2 hemocyte types were distinguished by light microscopy: granulocytes and hyalinocytes (or agranulocytes) according to the presence or the absence of cytoplasmic granules.
Cheng (1981) presented a morphological scheme based on numbers of cytoplasmic granules, dividing cells into 2 types: granulocytes, cells containing granules that ranged from very few to numerous; and agranulocytes, cells containing few or no granules.
After the separation in this type of density gradient, the granulocytes were separated from agranulocytes and pure granulocytes were obtained.