agarose gel electrophoresis

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Agarose Gel Electrophoresis

A technique in which large biomolecules are separated on a highly purified agarose gel by electrophoresis. AGE is used in clinical chemistry to separate mixtures of proteins by charge and size, and in molecular biology to separate mixtures of nucleic acid (DNA and RNA) fragments by sieving (movement of molecules through the gel’s pores) and size, where shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through gel pores.

agarose gel electrophoresis

see GEL ELECTROPHORESIS.
References in periodicals archive ?
Extracted DNA was visualized using 1% agarose gel to ensure its quantity and quality.
Isolated DNA samples were assessed by DNA spectrometry and 1 % agarose gel electrophoresis for their qualitative and quantitative analysis.
Keywords: Agarose gel, dsDNA, Silver staining protocol.
Empirical Bioscience's Pure Agarose Gel Extraction Kit is designed to quickly isolate small DNA fragments (70 bp to 20 kb) from agarose gel in just 15 minutes.
Agarose gel electrophoresis was carried out in a horizontal submarine electrophoresis unit.
The second step involves the gelling of the microemulsion drops to obtain agarose gel particles dispersed in n-heptane.
Amplified PCR products of 250 bp were resolved on 1.2% of agarose gel and visualized by gel documentation system (Fig.1).
All variants were genotyped by PCR and agarose gel electrophoresis.
Afterwards, they were trained in techniques such as the Polymerase Chain Reaction (PCR), Agarose Gel Electrophoresis as well as Restriction Digestion of DNA molecules.