agarose gel electrophoresis

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Agarose Gel Electrophoresis

A technique in which large biomolecules are separated on a highly purified agarose gel by electrophoresis. AGE is used in clinical chemistry to separate mixtures of proteins by charge and size, and in molecular biology to separate mixtures of nucleic acid (DNA and RNA) fragments by sieving (movement of molecules through the gel’s pores) and size, where shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through gel pores.

agarose gel electrophoresis

see GEL ELECTROPHORESIS.
References in periodicals archive ?
A kit for the separation and quantitation of serum proteins by the agarose gel electrophoresis method with an evaluation on a solar densitometer
The genomic DNA was run in 1% agarose gel, PCR product was run in 2% agarose gel and digestion products were run on 1.
The amplified product (223 bp) was electrophoresed in 2% agarose gel stained with ethidium bromide (0.
All variants were genotyped by PCR and agarose gel electrophoresis.
Afterwards, they were trained in techniques such as the Polymerase Chain Reaction (PCR), Agarose Gel Electrophoresis as well as Restriction Digestion of DNA molecules.
RNA extractions using these methods were analysed using a spectrophotometer and run on an agarose gel, followed by RT-PCR and sequencing.
The PCR amplicon was purified by agarose gel electrophoresis and then ligated into PCR 2-TOPO vector.
The samples were frozen and stored on ice at -20[degrees]C prior to use for rotavirus agarose gel electrophoresis and PCR genotyping.