Affinity Tag

Affinity Tag

A short peptide deliberately added to either the N- or C-end of a recombinant protein to facilitate purification of the expressed protein.
References in periodicals archive ?
The topics include mass spectrometry utilizing isotope-coded affinity tag reagents, the proteome in neurodegenerative diseases, the proteome related to respiratory disease, hepatitis C virus infection and mitochondria proteomics, and the quantitative proteomic analysis of HIV infection.
However, conventional affinity tag systems include large protein tags such as green-fluorescent proteins (9 ~ > 27kDa), FLAGs with specific antibodies (9 ~ > 150kDa), an d quantum dots (> ~ 20 [Angstrom]), (1,2) and they affect the affinity to bio-molecules.
In the first laboratory period, each pair of students receives a bacterial lysate containing a pair of unknown fluorescent proteins, one of which possesses an affinity tag.
For example, the protein samples are mixed with the ICAT reagents, which consist of a cysteine reactive moiety, a hiotin affinity tag for isolation and purification, and a linker region consisting of hydrogens (light tag) or deuteriums (heavy tag).
The first five chapters examine the production of proteins for structural biology, specifically discussing the use of a dual affinity tag for the production of recombinant protein; the cloning, expression, and purification of yeast proteins in a medium-sized structural genomics group, baculovirus expression of integral membrane proteins, and selenomethionine substitution in bacteria and eukaryotic cells.
Complementary analysis of the Mycobacterium tuberculosis proteome by two-dimensional electrophoresis and isotope coded affinity tag technology.
Oxford GlycoSciences entered a five-year collaboration to develop an industrial-scale proteomics platform using the Institute for Systems Biology's Isotope Coded Affinity Tag (ICAT) method .
This novel service is based on a revolutionary new platform, combining affinity tag binding with inert porous materials, designed especially for biocatalysis application in any organic solvent or aqueous buffer.
Chapters describe studies of protein-protein interactions using near-infrared fluorescence, nuclear magnetic resonance, and mass spectrometry; protein-ligand interactions using mass spectrometry, surface plasmon resonance, nuclear magnetic resonance, and isotope-coded affinity tag labeling; and structural proteomics using nuclear-magnetic resonance, x-ray crystallography, and electron paramagnetic resonance.
hp vectors are available for intracellular expression, without or with affinity tag for convenient purification: