This suggested that the affinity adsorbent bound trypsin more tightly when the acidic solution was added.
Even the product-type peptides were found effective for the preparation of affinity adsorbent for trypsin if chromatography is carried out at pHs lower than the optimum pH of catalysis.
In FAC, a relatively large volume of solution containing a substance for examination (analyte) is applied continuously to a relatively small column packed with affinity adsorbent (Fig.
We prepared a weak affinity adsorbent for ConA by immobilizing p-aminophenyl-[beta]-D-glucoside on Sepharose, and measured the extent of retardation of ConA after the passage through a column packed with the adsorbent.
Affinity adsorbent was prepared by using specially manufactured fine agarose gel beads tolerable against a high flow rate (HiTrap gel).
In FAC, a relatively large volume of dilute analyte solution is continuously applied to a small column packed with an affinity adsorbent, which contains immobilized ligands (Fig.
It was reported on Friday that the contract has been signed for the development of an affinity adsorbent
and associated purification process for the production of a novel vaccine product.
2 February 2010 - Danish biotechnology company Novozymes A/S (CPH: NZYM B) and Canadian biopharmaceutical company ProMetic Life Sciences Inc (TSE: PLI) said today they have formed a strategic alliance regarding proprietary albumin purification technology based upon a synthetic-ligand affinity adsorbent developed by ProMetic's UK subsidiary, ProMetic BioSciences Ltd.
Under the terms of the co-marketing alliance, the companies will jointly promote the use of a new synthetic-ligand affinity adsorbent, marketed under the trade-name AlbuPure, as a platform approach for the purification of albumin and albumin-fusion proteins.
Under the agreement, ProMetic will develop a Mimetic Ligand affinity adsorbent
for this new client as well as associated process conditions for the manufacturing of the client's next generation of its recombinant biopharmaceutical product.
PlasmaCap EBA uses proprietary affinity adsorbents
in expanded bed adsorption chromatography to capture plasma proteins directly from plasma or fractionated plasma materials without the use of precipitating solvents such as ethanol.
Chromatographic performance of the lectin affinity adsorbents
Adsorbent Ligand density, Glycoprotein sorption mg [mL.sup.-1] capacity mg [mL.sup.-1] %, of max * PEHA(1.1)-Cel(WGA) 18.5 2.6 10.5 OXY-Cel(WGA) 17.5 1.3 8.7 PEHA(0.37)-Cel(ConA) 7.5 7.4 62.9 OXY-Cel(ConA) 9.0 3.1 22.0 Adsorbent Recovery, % PEHA(1.1)-Cel(WGA) 66 OXY-Cel(WGA) 70 PEHA(0.37)-Cel(ConA) 93 OXY-Cel(ConA) 70 * Theoretical maximum sorption capacity was calculated from the ligand density considering one binding site per ligand molecule.